Screening method and application of human colon cancer cell specific binding polypeptide
A technology of combining human colon cancer cells and peptides, which is applied in the medical field, can solve problems such as low penetration, high price, and toxic effects, and achieve the effect of overcoming immune allergic reactions and small molecular weight
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[0019] 1. Screening method:
[0020] The screening method for specific binding polypeptides on the surface of colon cancer cells in this embodiment includes the following steps:
[0021] Step (1): Using the whole-cell screening method, incubate the random twelve-peptide library phage clones with colon cancer cells, discard the clones that are not combined with the colon cancer cells, and combine with the colon cancer cells Expansion of bound clones;
[0022] Step (2): Cycle step (1) 4 times to maximize the enrichment of phage clones that bind to the colon cancer cells to obtain clones that bind to colon cancer cells with a higher affinity and perform amplification;
[0023] Step (3): Incubating the above-mentioned amplified clones with normal colonic epithelial cells, and performing negative selection to remove non-specific clones combined with normal cells.
[0024] Step (4): Perform sequence determination on the final amplified clone, and analyze the polypeptide sequence. ...
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