Method for extraction, separation and histochemical localization of flavones in seven-color bamboo culm

Abstract

The invention provides a method for extraction and separation of flavones from a seven-color bamboo culm. According to the invention, total components are extracted from a seven-color bamboo culm raw material by utilizing a reflux extraction method; a crystal compound is separated out by utilizing silica gel column chromatography; six compounds contained in the seven-color bamboo culm are obtained through TCL thin-layer identification and spectrum resolution; and content determination is further performed. The invention provides an effective method for more comprehensively learning composed distribution of flavonoids in a bamboo plant through histochemical localization of flavones. The method comprises the following steps: carrying out histochemical localization for the flavones in a bamboo plant culm by utilizing a freehand section technology so as to learn the distribution condition of the flavonoids in the bamboo plant culm, thereby providing a basis for research of the metabolic process of various substances; then extracting chemical components from a bamboo culm, carrying out separation by utilizing silica gel column chromatography so as to obtain the crystal compound, and carrying out TCL thin-layer identification and spectrum resolution so as to obtain compounds contained in the bamboo culm; and carrying out high-performance liquid chromatography so as to obtain chemical component diagrams of seven-color bamboo in three stages, and accurately determining the content and the variety of the flavones in different stages.

Description

[0001] Technical field: [0002] The invention belongs to the field of chemical components of bamboo plants, and in particular relates to a method for extraction, separation and histochemical positioning of flavonoids in colorful bamboo stalks. [0003] Background technique: [0004] Bamboo plants are widely distributed. Preliminary research has been done on chemical composition, nutrition and health care, pharmacological effects, food development, etc. Bamboo plants are tree species with dual benefits of ecology and economy, and have high development value. Studies in recent years have shown that bamboo plants contain a large amount of flavonoid compounds, which are also the main physiologically active components in bamboo plants. Flavonoids have physiological functions such as scavenging free radicals, anti-inflammation, preventing cardiovascular diseases, anti-aging and lowering blood lipids, and have broad application prospects in health care products, medicine, food and ot...

AI Technical Summary

Problems solved by technology

For example: there are many studies on the chemical components of bamboo plants, but the research on the distribution and localization of flavonoids in bamboo plants is very lacking; there is also a lack of differences in the content of flavonoids in different parts of bamboo plants. A more comprehensive study; there is no relevant report on the synthesis rules and synthesis sites of flavonoids in bamboo plants and the impact of physiological stress on their formation.

Images