Implementation method for culture in plant culture bottle

Abstract

The invention discloses an implementation method for culture in a plant culture bottle and belongs to the technical field of plant culture. The implementation method for culture in the plant culture bottle comprises the following specific steps: S1: cutting and washing a plant material; S2: soaking the surface of the plant material for sterilization; S3: carrying out scrubbing with a disinfectant; S4: preparing a test culture medium; S5: sterilizing the test culture medium; S6: inoculating the test culture medium with plants; and S7: carrying out bottle capping, and carrying out cloth bag covering for culture. According to the method, different culture conditions can be provided according to different requirements of different parts of different plants, so that the growth cycle is relatively short; plant culture is carried out in the culture bottle, and thus, the management is convenient; and strict sterilizing treatment is carried out during culture, so that miscellaneous labor such as intertill-weeding, watering, fertilizing and disease and insect pest control is avoided, and thus, manpower and material resources are greatly economized.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for realizing cultivation in a plant cultivation bottle. Background technique [0002] Plant tissue culture is a new technology of asexual reproduction developed in recent decades based on the theory that plant cells are totipotent. In a broad sense, plant tissue culture is also called in vitro culture, which refers to the separation of tissues, organs or cells, protoplasts, etc. that meet the needs from the plant, and inoculation of various nutrients and plant hormones under aseptic conditions through aseptic operations. A technique for culturing on a given medium to obtain regenerated whole plants or to produce other products of economic value. In a narrow sense, tissue culture refers to the cultivation of various parts of plants, such as cambium, parenchyma, mesophyll tissue, endosperm, etc., to obtain regenerated plants, and also refers to the cultivation ...

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Problems solved by technology

[0003] The object of the present invention is to provide a method for realizing the cultivation of plants in a cultivation bottle, so as to solve the problem that the user cannot know the growth state of the plants in the cultivation bottle in time when the plants proposed in the background technology are cultivated in the cultivation flask, and artificial cultivation is required. For the plants in the culture bottle, the success rate of cultivation is relatively low

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