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Multi-target siRNA molecule targeting ems1/cortactin and its application

A multi-target, targeted technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, microbial measurement / inspection, etc., can solve the problems that the specific mechanism has not been studied clearly, and achieve the solution of poor silencing effect, accurate effect, Good effect on stability and silence effect

Active Publication Date: 2019-08-06
NANTONG UNIV XINGLIN COLLEGE +2
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Problems solved by technology

There are many literature reports about the close relationship between EMS1 / cortactin and HCC migration and invasion, but the specific mechanism has not been studied clearly

Method used

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  • Multi-target siRNA molecule targeting ems1/cortactin and its application
  • Multi-target siRNA molecule targeting ems1/cortactin and its application
  • Multi-target siRNA molecule targeting ems1/cortactin and its application

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Embodiment Construction

[0034] For convenience, in the following, the terms "siRNA", "siRNA sequence" or "siRNA molecule" are interchangeable, and they have the same meaning and scope. Among them, siRNA is a double-stranded structure formed by annealing the sense strand and the antisense strand. The siRNA molecules of the present invention are derived from the functional conserved regions of the open reading frame of the EMS1 / cortactin gene. The preparation of siRNA can use a variety of methods, such as: chemical synthesis, in vitro transcription, digestion of long-chain dsRNA, vector expression of siRNA, PCR synthesis of siRNA expression elements, etc. The emergence of these methods provides researchers with a choice. Better obtain gene silencing efficiency.

[0035] The present invention adopts a chemical synthesis method to prepare siRNA molecules, which can be used for HCC gene chip detection, EMS1 / cortactin expression to study the molecular mechanism of HCC transfer regulation, and can also be use...

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Abstract

The invention belongs to the technical field of siRNA molecule of targeting tumour-related gene, and relates to an EMS1 / cortactin-targeted multitarget siRNA molecule, and applications thereof. The EMS1 / cortactin-targeted multitarget siRNA molecule comprises following sequences: EMS1 / cortactin siRNA-5 sense strand and antisense strand, EMS1 / cortactin siRNA-6 sense strand and antisense strand, and EMS1 / cortactin siRNA-7 sense strand and antisense strand. Expression of EMS1 / cortactin is inhibited via transfection of human liver cancer (HCC)SMMC-7721 cell line, gene level is about 80%, down-regulating level is relatively stable and accurate. The multitarget siRNA is adopted in HCC gene chip detection, research of EMS1 / cortactin expression on HCC transferring regulating molecular mechanism, and preparation of EMS1 / cortactin-targeted anti-metastasis nucleic acid drugs.

Description

Technical field [0001] The invention belongs to the technical field of siRNA molecules targeting tumor-related genes, and relates to multi-target siRNA molecules targeting EMS1 / cortactin and applications. Background technique [0002] Primary liver cancer (HCC) is a complex and heterogeneous tumor with multiple genetic changes. Most patients die from postoperative recurrence and distant metastasis. The aggressiveness of liver cancer cells plays an important role in the recurrence and distant metastasis of HCC. Studies have shown that there is a 0.2-0.5 micron thick transparent area under the cell membrane, called the cell cortex. This layer structure is mainly composed of actin microfilaments cross-linked each other, which is closely related to important physiological activities such as cytoskeleton reorganization, cell movement, signal transduction, etc. [1] . The EMS1 gene is located on human chromosome 11q13, and its encoded protein has the ability to bind to actin in the co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12Q1/6886
CPCC12N15/113C12N2310/141C12Q1/6886C12Q2600/178
Inventor 周家名陈莉仲晶飞王桂兰吴美华
Owner NANTONG UNIV XINGLIN COLLEGE
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