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Micro-nano tissue engineering scaffold with immune treatment function and preparation method thereof

A technology of tissue engineering scaffold and immunotherapy, which is applied in the field of medical materials, can solve the problems of antibody inactivation, low loading, difficult antibody and high-efficiency immunotherapy, etc., and achieve the effect of immune regulation, inhibition of tumor promotion, and inhibition of tumor cell growth effects

Active Publication Date: 2017-07-04
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of carrying antibodies by electrospinning is mainly surface adsorption, and these loadings have disadvantages such as low loading capacity, low activity retention efficiency, and post-sterilization processes that easily inactivate antibodies.
Therefore, it is difficult to achieve efficient immunotherapy of antibodies

Method used

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  • Micro-nano tissue engineering scaffold with immune treatment function and preparation method thereof
  • Micro-nano tissue engineering scaffold with immune treatment function and preparation method thereof
  • Micro-nano tissue engineering scaffold with immune treatment function and preparation method thereof

Examples

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Embodiment 1

[0035] A method for preparing a micro-nano tissue engineering scaffold with immunotherapy function, comprising the following steps: (1) Preparation of electrospun fiber scaffold: dissolving PCL in a mixture of dichloromethane and N'N-dimethylformamide In the solution, wherein the mass ratio of PCL: dichloromethane: N'N-dimethylformamide is 0.2:1.5:10, after the dissolution is complete, electrospinning is carried out by an electrospinning device to prepare a PCL electrospinning fiber support; ( 2) Plasma surface modification of electrospun fiber scaffolds: PCL electrospun fiber scaffolds were subjected to plasma treatment, and the chamber pressure of the plasma processor was increased to 10 -3 Torr, inject oxygen and gaseous acrylic acid at 0.2Torr, and apply radio frequency power of 60W and negative pulse voltage for 30s to obtain carboxyl-functionalized PCL electrospun fiber scaffolds; (3) Grafting of antibodies: the surface modified PCL electrostatic Wash and dry the spun fi...

Embodiment 2

[0037] A method for preparing a micro-nano tissue engineering scaffold with immunotherapy function, comprising the following steps: (1) Preparation of electrospun fiber scaffold: dissolving PCL in a mixture of dichloromethane and N'N-dimethylformamide In the solution, wherein the mass ratio of PCL: dichloromethane: N'N-dimethylformamide is 5:15:1, after the dissolution is complete, electrospinning is carried out by an electrospinning device to prepare a PCL electrospinning fiber support; ( 2) Plasma surface modification of electrospun fiber scaffolds: PCL electrospun fiber scaffolds were subjected to plasma treatment, and the chamber pressure of the plasma processor was increased to 10 -3 Torr, inject oxygen and gaseous acrylic acid at 0.3 Torr, and apply radio frequency power of 50W and negative electrode pulse voltage for 40s to obtain carboxyl-functionalized PCL electrospun fiber scaffolds; (3) Grafting of antibodies: the surface modified PCL electrostatic Wash and dry the ...

Embodiment 3

[0039] A method for preparing a micro-nano tissue engineering scaffold with immunotherapy function, comprising the following steps: (1) Preparation of electrospun fiber scaffold: dissolving PLGA in a mixture of dichloromethane and N'N-dimethylformamide In the solution, the mass ratio of PLGA: dichloromethane: N'N-dimethylformamide is 0.5:3:4, and the silane coupling agent KH550 is added after the dissolution is complete, wherein the mass of the silane coupling agent is PLGA 5wt%, continue to stir evenly, and perform electrospinning through an electrospinning device to prepare PLGA electrospun fiber scaffolds; (2) Surface modification of coupling agent for electrospun fiber scaffolds: soak PLGA electrospun fiber scaffolds in hexamethylenediamine / n-propanol mixed solution at 25°C for 10 minutes, in which the mass ratio of ethylenediamine and n-propanol is 0.2:10, washed repeatedly with ethanol and deionized water and dried in vacuum to obtain amino-functionalized PLGA electrospu...

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Abstract

The invention provides a micro-nano tissue engineering scaffold with an immune treatment function and a preparation method thereof. The scaffold with the immune treatment function is prepared by surface activation modification and grafting of the surface of an electrostatic spun fiber scaffold with an antibody. The preparation method comprises the following steps: (1) preparation of the electrostatic spun fiber scaffold; (2) surface activation modification of the electrostatic spun fiber scaffold; (3) grafting with the antibody. Through grafting of the surface of the electrostatic spun scaffold with the antibody, on one hand, apoptosis of tumor cells is directly induced by antibody release, at the same time, antibody release can induce activation of dendritic cells in a tumor microenvironment, specific immune response is stimulated, cytokines are released and killing T cells are activated to kill tumor, and the tumor cell growth effect is indirectly inhibited; on the other hand, the scaffold after the antibody is released provides a solid carrier for tissue regeneration, and induces regenerated tissue regeneration. Finally, double functions of the biological scaffold in immune regulation inhibition of the tumor and promotion of regeneration of new tissues are achieved.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a micro-nano tissue engineering scaffold with immunotherapy function and a preparation method thereof. Background technique [0002] Antibody-based immunotherapy refers to a method of regulating the body's immune system through antibodies, enhancing or inhibiting the immune response ability, and treating diseases. Tumor immunotherapy can control and kill tumor cells by mobilizing the body's immune system and enhancing the anti-tumor immunity of the tumor microenvironment. Conversely, the suppression of immune responses by specific antibodies to treat autoimmune diseases is also an important area of ​​antibody application. At present, antibody-targeted therapy has been widely used in leukemia, gastric cancer, lung cancer and other tumor patients and rheumatoid arthritis patients. Compared with chemotherapeutic drugs, antibody treatment of tumors has the advantages of good specif...

Claims

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Application Information

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IPC IPC(8): A61L27/28A61L27/18A61L27/54A61L27/50C08J7/12C08L67/04
CPCA61L27/18A61L27/28A61L27/50A61L27/54A61L2300/256A61L2300/416A61L2300/426A61L2300/602C08J7/12C08J2367/04
Inventor 施勤崔文国刘星志赵环顾巧丽倪莉周熙超朱雪松
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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