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Detecting method for accurate risk early warning and accurate drug use of cardiovascular and cerebrovascular diseases and specific primer

A technology of cardiovascular and cerebrovascular diseases and primers, applied in the biological field, achieves high specificity, is suitable for popularization and application, and reduces experimental costs

Active Publication Date: 2017-06-27
李爱娟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology has not been applied to the precise drug gene detection of aspirin resistance and nitroglycerin resistance.

Method used

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  • Detecting method for accurate risk early warning and accurate drug use of cardiovascular and cerebrovascular diseases and specific primer
  • Detecting method for accurate risk early warning and accurate drug use of cardiovascular and cerebrovascular diseases and specific primer
  • Detecting method for accurate risk early warning and accurate drug use of cardiovascular and cerebrovascular diseases and specific primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Embodiment 1. The method and special primers for detecting drug resistance SNP sites of cardiovascular and cerebrovascular diseases

[0118] 1. Special primers for detecting drug resistance SNP sites of cardiovascular and cerebrovascular diseases

[0119] According to the key SNP sites of genes related to hypertension, coronary heart disease, dyslipidemia, and Alzheimer's disease, aspirin resistance, nitroglycerin resistance, individual differences in warfarin, and related SNP sites of genes related to clopidogrel resistance: rs5918, rs1330344, rs20417, rs671, rs1057910, rs9923231, rs4244285, rs4986893, rs1801133, rs3798220, rs2303790, rs429358, rs7412, design specific PCR primers and UEP primers, and use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology to achieve heart Drug resistance gene detection for cerebrovascular diseases.

[0120] Specific PCR primers and UEP primers include:

[0121] rs5918PCR primer 1: AC...

Embodiment 2

[0193] Example 2, the method for detecting drug resistance SNP sites of cardiovascular and cerebrovascular diseases and the application of special primers

[0194] 1. Extraction of template DNA

[0195] The peripheral blood of 5 subjects was used as samples 1-5 to be tested, and DNA was extracted according to the method of 2-1 of Example 1. Subjects 1-5 all had symptoms such as high blood pressure and thrombus; subjects 6-8 Aged 60-65 years old, without hypertension, coronary heart disease, senile dementia, dyslipidemia and other normal controls.

[0196] 2. PCR reaction: same as 2 of embodiment 1.

[0197] 3. Alkaline phosphatase treatment (SAP digestion reaction): the same as 3 of 2 of Example 1.

[0198] 4. Single base extension reaction: the same as 4 of 2 of Example 1.

[0199] 5. Resin purification: the same as the second part of 5 of embodiment 1.

[0200] 6. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) detection:

[0...

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Abstract

The invention discloses a detecting method for accurate risk early warning and accurate drug use of cardiovascular and cerebrovascular diseases and a specific primer. The invention provides a specific primer for detecting SNP site of drug resistance of the cardiovascular and cerebrovascular diseases, which comprises a primer group 1 and a primer group 2; the primer group 1 is composed of primer pair 1-primer pair 13; a primer group 2 is composed of single stranded extended primer 1- single stranded extended primer 13; the method can efficiently detect and complete the accurate early warning of the attack of the cardiovascular and cerebrovascular diseases and the accurate drug use after being attacked, namely, four kinds of cardiovascular and cerebrovascular disease early warning with huge health risk and accurate drug use of four clinical common drugs (aspirin, nitroglycerin, warfarin, and clopidogrel) for prevention and treatment of cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection method and special primers for precise early warning of cardiovascular and cerebrovascular disease risks and precise drug use. Background technique [0002] Cardiovascular and cerebrovascular diseases in my country have the characteristics of high prevalence and high mortality, and the harm is huge. It is of great significance to strengthen the health management of cardiovascular and cerebrovascular diseases, realize early detection and early warning of diseases, and carry out precise drug treatment according to individual genetic characteristics. [0003] Precision medicine is the best way to prevent and treat diseases based on individual genetic characteristics, environment and living habits. Precise early warning of diseases and precise medication are important components of precision medicine. Individuals carry different genetic single nucleotide polymorphisms, thei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 李爱娟谢文博赵菁
Owner 李爱娟
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