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Adenosine deaminase detection reagent

A technology for adenosine deaminase and detection reagents, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as hindering actual clinical use, high serum absorbance, and inconvenient clinical application, so as to facilitate popularization and application , High measurement precision, stable reagent effect

Inactive Publication Date: 2017-06-27
徐淼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is also useless due to the interference of serum ammonia and non-specific oxidation caused by excessive NADPH in the test system.
For the third-generation ADA test method, the absorbance of serum at 293nm is too high, which makes it inconvenient for clinical application
The fourth-generation ADA test This method overcomes the difficulties of previous ADA tests, however, due to the high cost of reagents, it hinders practical clinical use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The composition of the adenosine deaminase detection reagent of this embodiment is: purine nucleoside phosphorylase 6u / L; Glycine buffer solution 20mmol / L; Mannitol 20mmol / L; Sodium benzoate 20mmol / L; Xanthine oxidase 10u / L L; disodium edetate 0.05g / L; glyceraldehyde 10g / L; sodium azide 0.3g / L; disodium hydrogen phosphate 20mmol / L; peroxidase 50u / L; KCl 80mmol / L; N -Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt 2mmol / L; preservative 0.4ml / L; adenosine 80mmol / L; 4-aminotipyrine 5mmol / L L; color reagent 20mmol / L; adenosine 50mmol / L; bovine serum albumin 6g / L; polyethylene glycol 60000.3g / L.

Embodiment 2

[0016] The composition of the adenosine deaminase detection reagent of this embodiment is: purine nucleoside phosphorylase 10u / L; Glycine buffer 40mmol / L; Mannitol 30mmol / L; Sodium benzoate 40mmol / L; Xanthine oxidase 20u / L L; disodium edetate 0.05g / L; glyceraldehyde 20g / L; sodium azide 0.3g / L; disodium hydrogen phosphate 100mmol / L; peroxidase 100u / L; KCl 90mmol / L; N -Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt 2mmol / L; preservative 0.5ml / L; adenosine 95mmol / L; 4-aminotipyrine 8mmol / L L; color reagent 30mmol / L; adenosine 70mmol / L; bovine serum albumin 8g / L; polyethylene glycol 6000 0.3g / L.

Embodiment 3

[0018] The composition of the adenosine deaminase detection reagent of this embodiment is: purine nucleoside phosphorylase 8u / L; Glycine buffer solution 30mmol / L; Mannitol 25mmol / L; Sodium benzoate 30mmol / L; L; disodium edetate 0.05g / L; glyceraldehyde 15g / L; sodium azide 0.3g / L; disodium hydrogen phosphate 60mmol / L; peroxidase 80u / L; KCl 85mmol / L; N -Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt 2mmol / L; preservative 0.45ml / L; adenosine 88mmol / L; 4-aminotipyrine 7mmol / L L; color reagent 25mmol / L; adenosine 60mmol / L; bovine serum albumin 7g / L; polyethylene glycol 6000 0.3g / L.

[0019] Using the adenosine deaminase detection reagent of the present invention to carry out test experiments on patients, it is found that compared with the existing test reagents, the test speed is faster, the accuracy is higher, the display is clearer, and it is easy to store and has a long shelf life; Moreover, the anti-interference ability is strong and more stable.

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Abstract

The invention relates to an adenosine deaminase detection reagent. The adenosine deaminase detection reagent contains the following components: 6u / L-10u / L of purin-nukleosid-phosphorylase, 20mmol / L-40mmol / L of a glycine buffer solution, 20mmol / L-30mmol / L of mannitol, 20mmol / L-40mmol / L of sodium benzoate, 10u / L-20u / L of xanthine oxidase, 0.05g / L of disodium ethylenediamine tetraacetic acid, 10g / L-20g / L of glyceraldehydes, 0.3g / L of sodium azide, 20mmol / L-100mmol / L of disodium hydrogen phosphate, 50u / L-100u / L of peroxidase, 80mmol / L-90mmol / L of KCl, 2mmol / L of N-ethyl N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, 0.4ml / L-0.5ml / L of a preservative, 80mmol / L-95mmol / L of adenosine, 5mmol / L-8mmol / L of 4-amino antipyrine, 20mmol / L-30mmol / L of a developing agent, 50mmol / L-70mmol / L of adenine nucleoside, 6g / L-8g / L of bovine serum albumin and 0.3g / L of polyethylene glycol 6000. The adenosine deaminase detection reagent has high determination precision and strong disturbance resistance and is suitable for automatic rapid determination.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection reagent, in particular to an adenosine deaminase detection reagent. Background technique [0002] Adenosine deaminase (ADA) is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Determination of ADA and its isoenzyme levels in blood and body fluids for the diagnosis, differential diagnosis, treatment and immune function research of certain diseases has been paid more and more attention in clinic. ADA activity is a sensitive indicator to reflect liver damage, and can be used as one of the routine liver function inspection items. The liver enzyme spectrum combined with ALT or GGT can more comprehensively reflect the enzyme changes of liver diseases. [0003] In the past ten years, with the in-depth research on ADA, the detection methods have also been continuously developed. Four generations have been devel...

Claims

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Application Information

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IPC IPC(8): C12Q1/48C12Q1/34C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/34C12Q1/48C12Q2326/96
Inventor 徐淼
Owner 徐淼
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