A kind of carbon quantum dot fluorescent labeling material and preparation method thereof
A carbon quantum dot and fluorescent labeling technology is applied in the field of carbon quantum dot fluorescent labeling material and its preparation, which can solve the problems of difficult separation, complicated operation and high reaction temperature, and achieves the effect of low cost
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Embodiment 1
[0034] A carbon quantum dot fluorescent labeling material and a preparation method thereof. The steps of the preparation method described in this embodiment are:
[0035] (1) According to coal-based pyrolysis carbon: concentrated HNO 3 The mass ratio is 1: (10~16), the coal-based pyrolytic carbon is placed in the reactor first, and then the concentrated HNO 3 Add it into the reaction kettle, stir and reflux at 90-100°C for 4-5 hours, cool to room temperature, and take it out to obtain a black liquid.
[0036] (2) Filter the black liquid with a filter membrane with a pore size of 0.22 μm to obtain a filtrate; add NaOH solution to the filtrate to adjust the pH value to 5-6 to obtain a brown-black liquid.
[0037] (3) According to the brown-black liquid: the volume ratio of absolute ethanol is 1: (3~4), add absolute ethanol to the brown-black liquid, ultrasonically oscillate for 4~5 hours; centrifuge for 30 minutes, Obtain the supernatant and sink, remove the supernatant, the ...
Embodiment 2
[0040] A carbon quantum dot fluorescent labeling material and a preparation method thereof. The steps of the preparation method described in this embodiment are:
[0041] (1) According to coal-based pyrolysis carbon: concentrated HNO 3The mass ratio is 1: (13~19), the coal-based pyrolytic carbon is placed in the reactor first, and then the concentrated HNO 3 Add it into the reaction kettle, stir and reflux at 95-105°C for 3-4 hours, cool to room temperature, and take it out to obtain a black liquid.
[0042] (2) Filtrate the black liquid with a filter membrane with a pore size of 0.22 μm to obtain a filtrate; add NaOH solution to the filtrate to adjust the pH value to 6-7 to obtain a brown-black liquid.
[0043] (3) According to the brown-black liquid: the volume ratio of absolute ethanol is 1: (3~4), add absolute ethanol to the brown-black liquid, ultrasonically oscillate for 4~5 hours; centrifuge for 30 minutes, Obtain the supernatant and sink, remove the supernatant, the...
Embodiment 3
[0046] A carbon quantum dot fluorescent labeling material and a preparation method thereof. The steps of the preparation method described in this embodiment are:
[0047] (1) According to coal-based pyrolysis carbon: concentrated HNO 3 The mass ratio of the carbon is 1: (16-22), the coal-based pyrolytic carbon is placed in the reactor first, and then the concentrated HNO 3 Add it into the reaction kettle, stir and reflux at 100-110° C. for 3-4 hours, cool to room temperature, and take it out to obtain a black liquid.
[0048] (2) Filter the black liquid with a filter membrane with a pore size of 0.22 μm to obtain a filtrate; add NaOH solution to the filtrate to adjust the pH value to 7-8 to obtain a brown-black liquid.
[0049] (3) According to the brown-black liquid: the volume ratio of absolute ethanol is 1: (4~5), add absolute ethanol to the brown-black liquid, ultrasonically oscillate for 4~5 hours; centrifuge for 30 minutes in a centrifuge, Obtain the supernatant and s...
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