Fluorescence quantitative PCR (Polymerase Chain Reaction) detection kit and detection method

Abstract

The invention discloses a fluorescence quantitative PCR (Polymerase Chain Reaction) detection kit. The kit comprises a fluorescence quantitative PCR detection part and a result treatment and evaluation part, wherein the result treatment and evaluation part comprises a detection result treatment system corresponding to loci and a reference database; a locus which is already amplified is detected through a peak diagram of a dissociation curve, so as to obtain the detection result of the corresponding locus, the detection result is subjected to result treatment, the risk point of each locus is subjected to statistics to subsequently obtain the risk value of a to-be-detected gene, and corresponding to a risk level in a database, the risk of the gene and the relevant treatment result are comprehensively obtained. According to the fluorescence quantitative PCR detection kit, through a multiple detection kit adopting a fluorescence quantitative PCR, the detection results of multiple genes can be evaluated; based on a scientific and accurate result reference database, a result which is quite accurate and is moreover extremely highly individualized can be obtained; individualized result and suggest can be given to a detected sample; the purpose of more effectively detecting the gene is already achieved; the fluorescence quantitative PCR detection kit has higher use and popularization value.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a gene detection and evaluation system, in particular to a fluorescent quantitative PCR detection kit and a detection method. Background technique [0002] With the continuous development of scientific research, after the completion of the Human Genome Project and the full development of the Post-Genome Project, the application of genetic engineering-led biotechnology in the field of sports issues has received widespread attention. At present, there has been preliminary progress in the selection of sports materials using the theory and methods of genetics. Commonly used gene detection methods include: direct sequencing (DS), ligase detection reaction (LDR), restriction fragment length polymorphism (RFLP), denaturing high performance liquid chromatography analysis (denaturing high performance liquid chromatography, DHPLC) and quantitative PCR, among which direct sequencing usi...

AI Technical Summary

Problems solved by technology

Most of the existing studies on sudden cardiac death genes are based on the research on the loci of ion channel-related genes. For the Chinese population, the correlation of the loci is weak, and it is difficult to obtain more accurate results through detection. Therefore, it is very necessary to establish an evaluation system for the Chinese population.

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