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Magnetic composite microspheres, and preparation method and application thereof

A magnetic composite microsphere and microsphere particle size technology, which is applied in the direction of microsphere preparation, biochemical equipment and methods, magnetic materials, etc., to improve enzyme activity recovery ability, temperature stability, good reusability, and temperature stability Improved effect

Active Publication Date: 2017-06-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the magnetic composite material Fe 3 o 4 @SiO 2 - p (GMA - co - NIPAM) preparation and immobilized cellulase, there is no literature report at home and abroad

Method used

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  • Magnetic composite microspheres, and preparation method and application thereof
  • Magnetic composite microspheres, and preparation method and application thereof
  • Magnetic composite microspheres, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Fe 3 O 4 Synthesis

[0045] Dissolve 1.350g of ferric chloride hexahydrate in 70mL of ethylene glycol, add 3.854g of ammonium acetate, 0.400g of trisodium citrate, stir for 1h at an oil bath temperature of 170℃ until the solution becomes a homogeneous system, and transfer to the PTFE lining The stainless steel reactor was heated to 200℃ for 16h. After taking it out, it was cooled to room temperature, the product was magnetically separated, washed with ethanol until the supernatant was transparent, and dried in a vacuum drying oven at 50°C to a constant weight.

Embodiment 2

[0046] Example 2: Fe 3 O 4 @ SiO 2 -Synthesis of p (GMA-co-NIPAM)

[0047] (1) Fe 3 O 4 @ SiO 2 -Synthesis of MPS

[0048] 0.1 g of the product Fe in Example 1 3 O 4 Disperse in 15 mL of a 4:1 volume ratio of ethanol and water mixed solution, then add 1 mL of ammonia solution, mechanically stir and slowly drop 0.5 mL of TEOS into the system, and react at room temperature for 6 hours. Add 0.3mL MPS into the system and continue to stir and react for 12 hours. The product was separated magnetically and washed several times with ethanol. Dry to constant weight in a vacuum drying oven at 50°C.

[0049] (2) Fe 3 O 4 @ SiO 2 -Synthesis of p (GMA-co-NIPAM)

[0050] Take 50mg Fe 3 O 4 @ SiO 2 -MPS in a single-neck flask, add 40mL acetonitrile ultrasonic dispersion for 3min, add 50mgNIPAM, 0.05mL GMA, 100mg crosslinker MBA, 4mg initiator AIBN, oil bath heating flask, install fractionation column, condenser, receiver. The reaction flask was heated to boiling within 30 minutes from roo...

Embodiment 3

[0051] Example 3: Fe 3 O 4 @ SiO 2 -Synthesis of p (GMA-co-NIPAM)

[0052] (1) Fe 3 O 4 @ SiO 2 -Synthesis of MPS

[0053] Add 0.5 g of the product Fe in Example 1 3 O 4 Disperse in 90 mL of a 4:1 volume ratio of ethanol and water mixed solution, then add 3 mL of ammonia solution, mechanically stir and slowly drop 1.2 mL of TEOS into the system, and react at room temperature for 24 hours. Add 1.5 mL MPS into the system, and continue to stir and react for 48 hours. The product was separated magnetically and washed several times with ethanol. Dry to constant weight in a vacuum drying oven at 50°C.

[0054] (2) Fe 3 O 4 @ SiO 2 -Synthesis of p (GMA-co-NIPAM)

[0055] Take 100mg Fe 3 O 4 @ SiO 2 -MPS in a single-necked flask, add 80mL acetonitrile ultrasonic dispersion for 3min, add 150mg NIPAM, 0.15mL GMA, 200mg crosslinker MBA, 10mg initiator AIBN, oil bath heating flask, install fractionation column, condenser, receiver. The reaction flask was heated from room temperature to...

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Abstract

The invention relates to magnetic composite microspheres, and a preparation method and application thereof, belonging to the technical field of chemical material preparation. The preparation method comprises the following steps: synthesizing Fe3O4@SiO2-MPS (modified polystyrene); mixing, adding acetonitrile, carrying out ultrasonic dispersion, adding isopropyl acrylamide, glycidyl methacrylate, N,N'-methylene-bis acrylamide and azodiisobutyronitrile, mixing, and carrying out oil-bath heating; distilling to obtain part of acetonitrile, and stopping the reaction; carrying out magnetic separation on the obtained, and repeatedly washing with ethanol and water; and drying in a vacuum drying oven to obtain the product. The product is used for cellulase immobilization. The synthesized magnetic composite microspheres have superparamagnetism, can enhance the enzyme activity restorability and heat stability when being used for cellulase immobilization, and implements thermal protection on the cellulase.

Description

Technical field [0001] The invention relates to a magnetic composite microsphere and a preparation method and application thereof, in particular to the synthesis of a temperature-sensitive magnetic core-shell microsphere and its application in the immobilization of cellulase, belonging to the technical field of chemical material preparation. Background technique [0002] One of the main components of plants is cellulose, which accounts for about 35%-50% of the dry weight of plants. Cellulose is the most widely distributed natural carbohydrate in the world. The effective development and utilization of cellulose in plants is of great significance, and can effectively alleviate problems such as energy crisis, environmental pollution, energy saving, emission reduction, and food shortage. At present, the total utilization of cellulose is less than 0.5% of the world's output, and a large amount of cellulose resources have not been effectively, rationally and widely used. At present, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/06B01J13/14H01F1/11C12N11/14C12N11/08
CPCB01J13/06B01J13/14C12N9/2437C12N11/08C12N11/14H01F1/112
Inventor 刘倩王赟伍晨韩娟戎军辉李程王蕾倪良
Owner JIANGSU UNIV
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