Microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application

A micro-ecological preparation, the technology of Vannabin, which is applied in the field of micro-ecological preparations and its preparation for inhibiting Vibrio, can solve problems such as drug residues, restricting the development of the shrimp farming industry, and farmers' economic losses

Inactive Publication Date: 2017-06-13
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the high temperature season of spring and summer every year, various acute infectious diseases often break out, such as nerve necrosis virus, iridescent virus, vibrio and other pathogenic bacteria, which cause a large number of deaths of shrimp seedlings and juvenile shrimp, bringing huge economic losses to farmers. , seriously restricting the development of the shrimp farming industry
According to statistics, the annual mortality rate of prawn seedlings is as high as 80%. At present, in order to maintain the breeding density and prevent disease outbreaks in the process of prawn farming, various antibiotics are blindly used in production, such as chloramphenicol, oxytetracycline, furazolidone, etc. As a result, serious drug residues in the body of farmed animals have brought potential harm to the health of farmed animals and humans.

Method used

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  • Microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 A kind of preparation of probiotics

[0048] The cultivation of the bacteria of each component in the probiotics:

[0049] Bacillus safensis (Bacillus safensis) 1A07588 deposit number CCTCC NO: M2013290 and Bacillus pumilus including Bacillus pumilus (Bacillus pumilus) 1A08151 deposit number CCTCC NO: M2013291 cultivation: the culture of starting strains Bacillus safensis and Bacillus pumilus Cultivate in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): 3% bean cake powder, 1.5% starch, corn Syrup 1%, Glucose 0.5%, (NH 4 ) SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation condition is 28~37℃, the aeration culture is 36-48h, the number of viable bacteria is ≥10 9 CFU / mL;

[0050] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservati...

Embodiment 2

[0056] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0057] 1. Materials and methods

[0058] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / ml.

[0059] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 repetitions in each group.

[0060] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.

[0061] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors and placed randomly under natural conditions.

[0062]5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥ 10 6 CFU / mL, no test product was added to the control group. O...

Embodiment 3

[0072] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3

[0073] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of live bacteria is more than 1 billion / ml.

[0074] 2. Experimental prawns: The prawns from seedlings to 30 days old are used throughout. Set up 3 experimental pools and 7 control pools.

[0075] 3. Test site: a prawn farm in Shentu Town, Zhangpu County.

[0076] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. It is used once every three days, and the dosage per mu of aquaculture water body is 0.5-1L, and it is doubled when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 at the same time and evenly spray on the feed surface after diluting with appropriate amount of water, mix and feed the p...

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Abstract

The invention provides a microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application, and relates to aquaculture. The microecologic preparation comprises at least one of bacillus safensis and bacillus pumilus, and at least one of lactobacillus plantarum and lactobacillus plantarum; when the microecologic preparation is in a form of microbial bacteria solution, the preparation method comprises the following step: mixing a bacillus safensis fermented solution, a bacillus pumilus fermented solution, a lactobacillus plantarum fermented solution and a lactobacillus plantarum fermented solution; at least one of vitamin C, fructo-oligosaccharide, mannitol, and the like is added as a protecting agent to the mixture. The microecologic preparation is applicable to the preparation of preparations for promoting the growing of litopenaeus vannamei, improving the immunity and disease resistance of the litopenaeus vannamei, preventing and controlling the diseases of the litopenaeus vannamei, increasing the survival rate, purifying culture water quality, and particularly preventing and controlling intestinal tract thickening, red-leg disease, eye-rot disease and white spot syndrome virus of the litopenaeus vannamei. The microecologic preparation is applicable to preparation of veterinary drugs, feed additives or water purifiers, and the like.

Description

technical field [0001] The invention relates to aquaculture, in particular to a microecological preparation for inhibiting Vibrio in Litopenaeus vannamei culture, a preparation method and application thereof. Background technique [0002] Since 2003, with the promotion of high-density farming mode, my country's shrimp farming industry has entered a period of rapid development. In 2006, the total amount of shrimp farming in my country was 1.24 million tons, accounting for 39.2% of the global farmed shrimp production, and it has remained the world's first for many years . However, the rapid development of intensive and high-density aquaculture has seriously damaged the ecological environment of aquaculture, and also produced a series of negative effects, including waste of feed resources, environmental deterioration, serious diseases, germplasm degradation, drug residues, etc. , especially disease problems are becoming more and more serious. Shrimp white spot virus, taura vir...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A01K61/59A23K10/18A23K50/80A61K35/747A61P31/04A61P37/04A61K35/742C12R1/07C12R1/25C12R1/23
CPCA61K35/742A61K35/747C02F3/34C12N1/20A61K2300/00Y02A40/81Y02A40/818
Inventor 邵宗泽单大鹏李旭林剑辉王丽萍
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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