Method for culturing euscaphis japonica new germ plasm through space mutation breeding and proliferation culture medium

A proliferation medium and space mutagenesis technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of low seedling emergence rate, restrictions on the production and utilization of Corvus japonica seedlings, failure to maintain excellent variation characteristics, etc. Problems, to achieve ease of operation, improve the frequency of somatic embryos and regenerated plants of S. arvensis, and amplify the effects of spatial mutagenesis

Inactive Publication Date: 2017-06-13
厦门市园林植物园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It usually takes 3 years from harvesting to sowing, and the seedling rate is low, and it can't maintain good variation characteristics.
The shortcomings of these sowing seedlings restrict the production and utilization of the seedlings of Corvus japonica

Method used

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  • Method for culturing euscaphis japonica new germ plasm through space mutation breeding and proliferation culture medium
  • Method for culturing euscaphis japonica new germ plasm through space mutation breeding and proliferation culture medium
  • Method for culturing euscaphis japonica new germ plasm through space mutation breeding and proliferation culture medium

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Embodiment 1

[0033] A method of space mutation breeding for cultivating new germplasm of Arvenia japonica. Select mature and plump Arvenia japonica seeds, carry the "Shenzhou 10" spacecraft into space for 15 days, carry out space mutation breeding, and after sowing seedlings, obtain annual For seedlings, select the second side branch bud eye, clean it with detergent, rinse it in running water for 10 minutes, immerse it in 75% ethanol for 30 seconds, rinse it three times with sterile water, immerse it in 0.1% mercuric chloride for 8 minutes, rinse it with sterile water for 5 minutes. Next, drain the water, cut off the 03-0.5cm part of the terminal section soaked by the drug, cut off the leaves, and inoculate in the improved MS+6-BA (1.0mg / L)+NAA (0.2mg / L)+white sugar (25g / L ) + agar (7g / L) culture medium to induce callus tissue, transfer to the same culture medium again after 30 days, wait for the plant to grow to 3-4cm high, select the robust rootless seedlings and inoculate them into the r...

Embodiment 2

[0035] A method of space mutation breeding for cultivating new germplasm of Arvenia japonica. Select mature and plump Arvenia japonica seeds, carry the "Shenzhou 10" spacecraft into space for 15 days, carry out space mutation breeding, and after sowing seedlings, obtain annual For seedlings, cut off the bud eye of the second lateral branch, rinse with running water for 15 minutes, immerse in 75% ethanol for 30 seconds, rinse with sterile water for 3 times, immerse in 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 times, drain the water, and cut off the branches. Cut off the part of the terminal segment 03~0.5cm soaked by the medicine, cut off the leaves, inoculate on the medium of improved MS+0.5mg / L 6-BA+0.1mg / L NAA+25g / L white sugar+7g / L agar to induce callus After 20 days, transfer to the medium of improved MS+1.0mg / L 6-BA+0.2mg / L NAA+25g / L white sugar+8g / L agar to obtain regenerated seedlings. After 25-30 days, the growth and quality characteristics of...

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Abstract

The invention provides a method for culturing euscaphis japonica new germ plasm through space mutation breeding and a proliferation culture medium, and relates to plant breeding. Ripe euscaphis japonica seeds are used as carrying materials to be subjected to space mutation through being carried in a return-type spacecraft; after the mutation is completed, seedling plants are cultured; the plant axillary buds are selected for tissue culture; the original bud points directly form seedlings without callusing; branches at the top end of the tissue culture seedlings are cut to culture plant regeneration seedlings, and the new germ plasm with beneficial mutation is obtained after the artificial screening. The proliferation culture medium for culturing euscaphis japonica through space mutation breeding is prepared from an improved MS culture medium, (0.5 to mg / L of 6-BA, (0.1 to 0.3)mg / L of NAA, (25 to 30)g / L of white sugar and (7 to 9)g / L of agar, and the pH value is 5.8 to 6.2. The improved MS culture medium is prepared from major nutrition elements, trace nutrition elements and organic reagent.

Description

technical field [0001] The invention relates to plant breeding, in particular to a method for cultivating new germplasm of Arvenia japonica through space mutation breeding and a proliferation medium. Background technique [0002] Euscaphis japonica (Thunb.) Kanitz is a small tree belonging to the genus Euscaphis japonica (Staphyleaceae). Evergreen in four seasons, gray-white bark, beautiful tree shape; in spring and summer, yellow-white flowers, gathered on the top of the branches, full of silver flowers; fruit ripens from September to October, bright red pods crack, and bears fruit until April of the following year , for as long as 7 to 8 months, it is a good tree species for viewing leaves, flowers and fruits in gardens. The seeds of Corvus cavus, known as chicken eyes and chicken gizzards in traditional Chinese medicine, have the effects of warming the middle and regulating qi, reducing swelling and pain, treating stomach pain, cold colic, diarrhea, prolapse of the anus,...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/08A01H4/00
CPCA01H1/06A01H1/08A01H4/00A01H4/001A01H4/008
Inventor 蔡邦平张秀英马丽娟张万旗陈熙
Owner 厦门市园林植物园
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