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An experimental method to demonstrate the relationship between hydrogen sulfide and gap junctions

A technique of gap junctions and experimental methods, applied in the biological field, can solve problems such as the relationship between hydrogen sulfide and gap junctions has not been proved

Inactive Publication Date: 2019-09-06
SHIHEZI UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] At present, in the prior art, there is no relevant report on the experimental method to prove the relationship between hydrogen sulfide and gap junctions

Method used

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  • An experimental method to demonstrate the relationship between hydrogen sulfide and gap junctions
  • An experimental method to demonstrate the relationship between hydrogen sulfide and gap junctions
  • An experimental method to demonstrate the relationship between hydrogen sulfide and gap junctions

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Embodiment Construction

[0023] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0024] Grouping: WKY group: normal Wistar Kyoto (Wistar-Kyoto, WKY) rats, male or female, 9 weeks old, intraperitoneally injected with the same amount of normal saline as in the SHR+H2S group every day for 8 weeks.

[0025] SHR group: Spontaneously hypertensive rats (spontaneously hypertensive rats, SHR), male or female, 9 weeks old, intraperitoneally injected with the same amount of normal saline as in the SHR+H2S group for 8 weeks.

[0026] NaHS treatment group: SHR, male or female, 9 weeks old, daily intraperitoneal injection of 56 μmol / kg body weight of NaHS (H 2 S donor) for 8 weeks.

[0027] 1 Animal blood pressure measurement Use a fully automatic non-invasive blood pressure measuring instrument for rats in each group to detect the systolic pressure of the tail artery in each group. When measuring, w...

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Abstract

The invention discloses an experiment method for proving connection relationship between hydrogen sulfide and a gap. The method comprises the following steps: blood pressure measurement of animals, preparation of a rat peripheral blood lymphocyte suspension and detection of peripheral blood T-lymphocyte subsets and Cx40 and Cx43. The relationship between the hydrogen sulfide and protein on T-lymphocyte is disclosed for the first time; the hydrogen sulfide is prompted to communicate through affecting gap junction among immune cells and release of inflammatory factors is adjusted, so that the experiment method plays an important role in improvement of target organ damages caused by hypertensive inflammation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an experimental method for proving the relationship between hydrogen sulfide and gap junctions. Background technique [0002] Peripheral blood lymphocytes are divided into three groups according to their biological function and cell surface antigen expression: T lymphocytes (CD3+), B lymphocytes (CD19+) and NK lymphocytes [natural killer cells (natural killer), CD3-CD16+ and / or CD56+]. T cells are divided into helper T cells (CD3+CD4+) and suppressor T cells (CD3+CD8+). The analysis of lymphocyte subsets is an important indicator for detecting cellular immunity and humoral immunity. It generally reflects the current immune function, state and balance level of the body, and can assist in the diagnosis of certain diseases, such as autoimmune diseases, immunodeficiency diseases, malignant For tumors, blood diseases, allergic diseases, etc., it is of great significance to...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/50
Inventor 李新芝马克涛彭珉
Owner SHIHEZI UNIVERSITY
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