Method for differentiating propolis and poplar gum

A technology of poplar and propolis, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of loss of function, easy hydrolysis of salicin, identification of propolis and poplar gum, etc., to achieve simple operation, high sensitivity, and efficient detection method Effect

Inactive Publication Date: 2017-05-31
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, salicin is easily hydrolyzed. If unscrupulous traders use chemical means to destroy it, the method will lose its original effect.
[0005] Many active components in propolis have antioxidant properties, and there is no report on using the antioxidant active components in propolis as reference substances to identify propolis and poplar gum

Method used

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  • Method for differentiating propolis and poplar gum
  • Method for differentiating propolis and poplar gum
  • Method for differentiating propolis and poplar gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The identification method of propolis and poplar gum comprises the steps:

[0036] (1) Dissolve 0.2g of propolis with ethanol at 20°C ultrasonically, then centrifuge, and after filtration, rotate the filtrate to a paste to obtain a propolis extract; dissolve 0.2g poplar gum with ethanol at 20°C ultrasonically, then centrifuge, After filtration, the filtrate was rotary evaporated to a paste to obtain poplar gum extract.

[0037] (2) The propolis extract and poplar gum extract in step (1) were prepared with methanol into a propolis extract analysis solution with a concentration of 1 mg / mL and a poplar gum extract analysis solution with a concentration of 1 mg / mL.

[0038](3) Inject 20uL of the propolis extract analysis solution and the poplar gum extract analysis solution obtained in step (2) into the on-line detection system of the high performance liquid chromatography-free radical detection system, the high performance liquid chromatography Detection condition setting...

Embodiment 2

[0041] The method for discriminating propolis and poplar gum, comprises the steps:

[0042] (1) 0.2g of propolis was ultrasonically dissolved in ethanol at 25°C, then centrifuged, and after filtration, the filtrate was rotary evaporated to a paste to obtain a propolis extract; 0.2g of poplar gum was ultrasonically dissolved in ethanol at 25°C, then centrifuged, After filtration, the filtrate was rotary evaporated to a paste to obtain poplar gum extract.

[0043] (2) The propolis extract and poplar gum extract in step (1) were prepared with methanol into a propolis extract analysis solution with a concentration of 1.6 mg / mL and a poplar gum extract analysis solution with a concentration of 1.6 mg / mL.

[0044] (3) Inject 20uL of the propolis extract analysis solution and poplar gum extract analysis solution obtained in step (2) into the on-line detection system combined with high performance liquid chromatography-free radical detection system, wherein the detection conditions of...

Embodiment 3

[0047] The distinguishing method of propolis and poplar gum is characterized in that, comprises the steps:

[0048] (1) Dissolve 0.2g of propolis with ethanol at 27°C sonicated, then centrifuge, filter and then rotatively evaporate the filtrate to a paste to obtain a propolis extract; dissolve 0.2g poplar gum with ethanol at 27°C sonicated, then centrifuge, After filtration, the filtrate was rotary evaporated to a paste to obtain poplar gum extract.

[0049] (2) The propolis extract and poplar gum extract in step (1) were prepared with methanol into a propolis extract analysis solution with a concentration of 3 mg / mL and a poplar gum extract analysis solution with a concentration of 3 mg / mL.

[0050] (3) Inject 20uL of the propolis extract analysis solution and the poplar gum extract analysis solution obtained in step (2) respectively into the online detection system of the high performance liquid chromatography-free radical detection system: the high performance liquid chroma...

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Abstract

The invention relates to a method for differentiating propolis and poplar gum. The method includes following steps: (1), ultrasonically dissolving propolis and poplar gum respectively, centrifugally filtering, performing rotary evaporation on filtrate to form paste to obtain propolis extract and poplar gum extract; (2), using methanol to respectively dilute the propolis extract and the poplar gum extract to obtain propolis analysis liquid and poplar gum analysis liquid; (3), respectively injecting samples of the propolis analysis liquid and the poplar gum analysis liquid into a high performance liquid chromatography-free radical detection system combined online detection system; (4), comparing chromatogram of propolis with chromatogram of the poplar gum to differentiate propolis and the poplar gum. By the method, an anti-oxidation active ingredient can be used as a comparison product, the defect that fingerprint spectra are low in repeatability is overcome, and the defect that the comparison product is unstable due to degrading and activity loss of the active ingredient can be avoided. The method is efficiency, visual, simple and convenient to operate and high in sensitivity and repeatability.

Description

technical field [0001] The invention belongs to the field of food and drug quality control methods, in particular to a method for identifying propolis and poplar gum. Background technique [0002] Propolis has attracted a lot of attention due to its wide range of biological activities, such as anticancer, antioxidative, antiinflammatory, antibacterial, and antifungal activities, and has been widely used in healthcare and other fields, however, the production of propolis is limited Due to the impact of bee species, glue harvesting methods and glue sources, the output is relatively low, and the supply has been in short supply. Because poplar bud extract and poplar propolis extract are very similar in composition, color, and smell, the phenomenon of using poplar gum (poplar bud extract) as propolis has occurred frequently in recent years. The current quality control of propolis mainly focuses on flavonoids and polyphenols, and these two types of components poplar bud extract a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 杨红丽叶意群林琳
Owner ZHEJIANG OCEAN UNIV
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