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Primer for detecting gene polymorphism of RUNX1 rs2268277 and PCR (Polymerase Chain Reaction) method of primer

A technology of runx1rs2268277 and gene polymorphism, applied in the field of medical biology, can solve unexplainable problems, achieve the effect of simple method and wide application prospect

Inactive Publication Date: 2017-05-31
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still adverse reactions in some patients that cannot be explained

Method used

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  • Primer for detecting gene polymorphism of RUNX1 rs2268277 and PCR (Polymerase Chain Reaction) method of primer
  • Primer for detecting gene polymorphism of RUNX1 rs2268277 and PCR (Polymerase Chain Reaction) method of primer
  • Primer for detecting gene polymorphism of RUNX1 rs2268277 and PCR (Polymerase Chain Reaction) method of primer

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Primers and PCR method for detecting RUNX1rs2268277 gene polymorphism

[0026] 1. Reagents and configurations required for the experiment

[0027] (1) DNA extraction reagents

[0028] 6mol / L NaI (Shanghai Sangon Bioengineering Technology Service Co., Ltd., Shanghai), chloroform, isopropanol, isoamyl alcohol, and ethanol are commercially available analytically pure products.

[0029] (2) PCR amplification reagents

[0030] Ex Taq® enzyme (Takara, Japan), synthetic primers (Shanghai Sangon Bioengineering Technology Service Co., Ltd., Shanghai).

[0031] (3) Reagents for agarose gel electrophoresis

[0032] Ordinary agarose (Biowest Company, Spain), low melting point agarose (Shanghai Bioengineering Technology Service Co., Ltd., Shanghai), 5×TBE electrophoresis buffer storage solution (0.45 mol / L Tris base, 0.45 mol / L boric acid, 0.01 mol / L L EDTA, adjust the pH to 8.0; Tris, boric acid, and EDTA were purchased from Shanghai Sangon Bioengineering Technology S...

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Abstract

The invention discloses a primer pair RUNXF / RUNXR for detecting a gene polymorphism site of RUNX1 rs2268277 by using a PCR (Polymerase Chain Reaction) method, wherein the sequences of the primer pair are as shown in SEQ ID NO.1 and SEQ ID NO.2 respectively. The invention further discloses the application of the primer pair RUNXF / RUNXR in detecting the gene polymorphism site of a biological sample IMPDH1 rs4731447, and a detection kit which is prepared from the primer pair and used for detecting the gene polymorphism site of the RUNX1 rs2268277. By adopting the primer pair and the PCR method of the primer pair disclosed by the invention, the gene type of the gene polymorphism site of the RUNX1 rs2268277 can be confirmed according to amplification electrophoresis results, the treatment effect and the side effect of a purinethol medicine can be predicted, the effectiveness and the security of the purinethol medicine administrated to a patient can be predicted on the basis of gene type analysis, clinical medicine administration can be instructed, and individual treatment on patients can be achieved.

Description

technical field [0001] The invention belongs to the field of medical biotechnology. More specifically, it relates to a primer for detecting RUNX1rs2268277 gene polymorphism and a PCR method thereof. Background technique [0002] Thiopurines (mercaptopurines) are widely used clinically. However, this class of drugs has a high incidence of adverse reactions, including bone marrow suppression, liver damage, gastrointestinal disturbances, pancreatitis, and influenza-like symptoms. [0003] Current research suggests that adverse reactions of mercaptopurines may be related to drug metabolism and genetic polymorphisms. Thiopurine methyltransferase (TPMT) and inosine triphosphate pyrophosphatase (ITPA) are metabolic enzymes of mercaptopurine drugs, among which TPMT is the key enzyme in the metabolism of mercaptopurine drugs, TPMT and There are obvious genetic polymorphisms in ITPA. However, there are still adverse reactions in some patients that cannot be explained. Contents o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/106
Inventor 黄民王雪丁管少兴郑洪朱霞周珊张财斌胡品津高翔郅敏晁康
Owner SUN YAT SEN UNIV
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