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Method for recognizing contribution proportion of kiwi fruit hybrid patients on filial generation genome

A hybrid parent and genome technology, applied in the field of plant genetic resource evaluation and biotechnology, can solve problems such as low efficiency of early selection of hybrid resources, and achieve the effects of saving manpower and material costs, high accuracy, and promoting early screening

Active Publication Date: 2017-05-31
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of low early selection efficiency of kiwifruit hybrid resources, and provide a method for identifying the contribution ratio of kiwifruit hybrid parents to the offspring genome, which can be used to predict the variation range and direction of traits of kiwifruit hybrid offspring, and promote the development of hybrid resources. early selection efficiency

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  • Method for recognizing contribution proportion of kiwi fruit hybrid patients on filial generation genome
  • Method for recognizing contribution proportion of kiwi fruit hybrid patients on filial generation genome
  • Method for recognizing contribution proportion of kiwi fruit hybrid patients on filial generation genome

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Embodiment 1

[0023] Embodiment 1: the method for identifying the contribution ratio of kiwifruit hybrid parents to the offspring genome of the present embodiment, the steps are as follows:

[0024] 1. If figure 1 Step ① Perform low-depth genome sequencing on the offspring of the hybrid parents and the distantly related groups.

[0025] Select the sample of kiwifruit hybrid parental offspring to be analyzed and a sample of kiwifruit outgroup that is relatively distant from all materials in the hybrid combination, extract about 5 μg of total genomic DNA from each sample, and then construct and provide it with the Illumina sequencing platform A small fragment library of about 180bp was constructed with the kit and sequenced on its Hiseq2000 platform. The total amount of sequencing data reached about 3.5Gb, that is, the kiwifruit genome with an overall sequencing depth of 5 times for each sample was obtained.

[0026] Sequence filtering was performed on the sequencing fragments using FastQ s...

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Abstract

The invention discloses a method for recognizing the contribution proportion of kiwi fruit hybrid patients on a filial generation genome. The method comprises the following steps of performing genome low-depth sequence testing on hybrid parents and filial generations and distant hybrid outgroup; performing sequence testing data reference genome comparison, and obtaining single basic group variation information; performing single basic group variation-based genome window division and log probability estimation; performing sub window maximum possible gene tree building and confidence interval estimation; performing gene tree level and filial generation genetic relationship statistics and genome contribution ratio prediction on the hybrid parents. The whole genome variation information is used for analyzing the evolution genetic relationship of the hybrid patients and the hybrid filial generation, so that the prediction of the hybrid filial generation characteristic properties realizes high accuracy; meanwhile, by using the method, the magnitude and the direction of the possibly existing phenotypic characteristic variation are predicted in the baby period of the hybrid filial generation formation; the early stage screening of the hybrid strains can be greatly promoted; the labor and material cost can be reduced; the resource mining and utilization efficiency can be greatly improved.

Description

technical field [0001] The invention belongs to the field of plant genetic resource evaluation and biotechnology, and specifically relates to a method and application for identifying the contribution ratio of kiwifruit hybrid parents to the genome of offspring, that is, to analyze the contribution ratio of parents to the genome of hybrid offspring in kiwifruit hybrid combinations, thereby forming Predict the variation range and direction of traits of hybrid progeny, improve the early screening efficiency of hybrid progeny, reduce land and labor costs, and promote the rapid breeding of new hybrid varieties. Background technique [0002] Crop hybrid breeding is an important means of improving germplasm resources and cultivating new varieties. Through hybridization, the excellent traits of both parents can be integrated and new cross-parent characteristics can be further produced, resulting in extensive variation in yield, ecological adaptability and resistance. Kiwi (Actinidia...

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Application Information

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IPC IPC(8): C12Q1/68G06F19/20
CPCC12Q1/6895C12Q2600/156G16B25/00
Inventor 刘义飞李大卫张琼黄宏文
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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