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Application of Arabidopsis thaliana SSCD1 gene mutation in regulating synthesis of jasmonic acid in plants

A technology of jasmonic acid and Arabidopsis, applied in the field of defense system

Inactive Publication Date: 2017-05-31
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the research and application of this gene in improving the content of jasmonic acid in plants.

Method used

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  • Application of Arabidopsis thaliana SSCD1 gene mutation in regulating synthesis of jasmonic acid in plants
  • Application of Arabidopsis thaliana SSCD1 gene mutation in regulating synthesis of jasmonic acid in plants
  • Application of Arabidopsis thaliana SSCD1 gene mutation in regulating synthesis of jasmonic acid in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Under short-day conditions, the SSCD1 mutant gene significantly up-regulated the expression of the jasmonic acid synthesis gene LOX2 and AOS in the mutant

[0017] (A) Cultivation of materials: Disinfect wild-type Arabidopsis thaliana Col-0 seeds and sscd1 mutant seeds with disinfectant (containing 20% ​​84 disinfectant and 0.01% TritonX-100) for 10 minutes, and wash with sterile water After 3-5 times, spread on the solid medium of MS (containing 1% sucrose), and vernalize under the low temperature condition of 4°C. After 3 days, they were placed in a long-day cultivation room for cultivation, and the specific cultivation conditions were as follows: light / dark cycle of 16h / 8h, temperature of 22°C, light intensity of 100umolm-2s-1, and relative humidity of 70%. After 7 days of growth, move the seedlings to a new sterile MS medium. During the transplanting process, care should be taken not to damage the seedlings, and at the same time, leave enough space to ensu...

Embodiment 2

[0029] Example 2 Under short-day conditions, the SSCD1 mutant gene can increase the content of jasmonic acid in plants

[0030] (A) Cultivation of materials: Disinfect wild-type Arabidopsis thaliana Col-0 seeds and sscd1 mutant seeds with disinfectant (containing 20% ​​84 disinfectant and 0.01% TritonX-100) for 10 minutes, and wash with sterile water After 3-5 times, spread on the solid medium of MS (containing 1% sucrose), and vernalize under the low temperature condition of 4°C. After 3 days, they were placed in a long-day cultivation room for cultivation, and the specific cultivation conditions were as follows: light / dark cycle of 16h / 8h, temperature of 22°C, light intensity of 100umolm-2s-1, and relative humidity of 70%. After 7 days of growth, move the seedlings to a new sterile MS medium. During the transplanting process, care should be taken not to damage the seedlings, and at the same time, leave enough space to ensure the normal growth of the seedlings. Continue to pl...

Embodiment 3

[0033] Example 3 Under short-day conditions, the SSCD1 mutant gene induces the expression of the downstream response genes PDF1.2, VSP2, and THI2.1 of the jasmonic acid defense pathway

[0034] (A) Cultivation of materials: Disinfect wild-type Arabidopsis thaliana Col-0 seeds and sscd1 mutant seeds with disinfectant (containing 20% ​​84 disinfectant and 0.01% TritonX-100) for 10 minutes, and wash with sterile water After 3-5 times, spread on the solid medium of MS (containing 1% sucrose), and vernalize under the low temperature condition of 4°C. After 3 days, they were placed in a long-day cultivation room for cultivation, and the specific cultivation conditions were as follows: light / dark cycle of 16h / 8h, temperature of 22°C, light intensity of 100umolm-2s-1, and relative humidity of 70%. After 7 days of growth, move the seedlings to a new sterile MS medium. During the transplanting process, care should be taken not to damage the seedlings, and at the same time, leave enough ...

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Abstract

The invention relates to application of Arabidopsis thaliana SSCD1 gene mutation in regulating synthesis of jasmonic acid in plants. The sequence of the SSCD1 mutant gene is disclosed as SEQ ID NO.2. The comparison of the jasmonic acid synthesis gene expression, jasmonic acid content and jasmonic acid defence pathway downstream response gene expression in wild-type Arabidopsis thaliana Col-0 and SSCD1 gene mutation Arabidopsis thaliana sscd1 under short day conditions detects that the mutation of the SSCD1 gene can influence the synthesis of jasmonic acid in the plant, change the jasmonic acid content in the plant, activate the jasmonic acid defence pathway and induce the expression of the jasmonic acid defence pathway downstream response gene. The gene can be modified to enhance the jasmonic acid content in the plant, thereby culturing the high-jasmonic-acid-content plant.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering application, and mainly relates to the ability of Arabidopsis SSCD1 gene mutation to regulate the synthesis of jasmonic acid under short-day conditions, thereby affecting the defense system participated and mediated by jasmonic acid in plants. Background technique [0002] From September to March of the next year, most parts of my country belong to the short-day period. And this period is the harvest season of crops. In order to ensure the yield and quality of crops, agricultural producers reduce the occurrence of pests and diseases by spraying pesticides. The demand for agricultural products is increasing day by day, and it is becoming more and more difficult for agricultural products sprayed with pesticides or chemical fertilizers to meet people's requirements. Therefore, in agricultural production, it is necessary to change the traditional method of relying on pesticides to prevent...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8243
Inventor 任春梅周舟支添添
Owner HUNAN AGRICULTURAL UNIV
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