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Aedes albopictus densovirus and its application

A technology of densovirus and Aedes albopictus, applied in Aedes albopictus densovirus and its application fields, can solve problems such as environmental pollution and mosquito drug resistance, and achieve the effect of low transportation and storage costs

Active Publication Date: 2020-08-18
陈晓光 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its outstanding effect, chemical vector control once occupied a dominant position in mosquito vector control. However, with the serious environmental pollution caused by traditional chemical control, the disadvantages such as easy to cause mosquito drug resistance are becoming more and more obvious. Mosquitoes The biological control is more and more favored by people

Method used

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  • Aedes albopictus densovirus and its application
  • Aedes albopictus densovirus and its application
  • Aedes albopictus densovirus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Isolation and Identification of Mosquito Densovirus AalDV-6

[0059] Materials and Methods:

[0060] Aedes albopictus densovirus AalDV-6 (Aedes albopictus densovirus AalDV-6), preserved in China Center for Type Culture Collection (CCTCC), preservation number: CCTCC V 201652. Date of deposit: November 29, 2016, place of deposit: Wuhan University, Wuhan, China.

[0061] The 0.01mM pH7.2 phosphate buffer saline PBS used in this experiment was purchased from American Life Biotechnology Co., Ltd., the viral genome extraction kit TaKaRa miniBEST Viral RNA / DNA Extraction Kit was purchased from Takara Company, and Thermo Scientific Maxima Hot Start Green PCR Master Mix enzyme was purchased from American ThermoFisher Company, T carrier was purchased from Promega Company, Trans1-T1 chemically competent cells were purchased from Beijing Quanshijin Biotechnology Co., Ltd. Primer synthesis and DNA sequencing were completed in Shanghai Yingwei Jieji Trading Co., Ltd.

[0...

Embodiment 2

[0068] Example 2 Proliferation of Aedes albopictus densovirus-6 in Aedes albopictus C6 / 36 cells

[0069] Materials and Methods:

[0070] The viral genome extraction kit TaKaRa miniBEST Viral RNA / DNA Extraction Kit used in this experiment was purchased from Takara Company, EcoRV restriction endonuclease was purchased from Thermo Fisher Company, and SuperReal PreMix Plus was purchased from Beijing Tiangen Biotechnology Co., Ltd.

[0071] C6 / 36 cells were subcultured to 25cm 2 Cell culture flask, after 24 hours, the cell coverage is about 70-90%, discard the medium, rinse the cells once with 1mL 28°C preheated PBS, add the AalDV-6 detected in Example 1, and supplement with 5% fetal bovine Serum 1640 medium to 5mL, placed in a biochemical incubator at 28°C. Collect 200 μL of cell suspension every day and store at -80°C until day 7. The remaining cell suspension was repeatedly frozen and thawed 3 times, centrifuged at 3750 rpm at 4°C for 10 minutes, and the supernatant was trans...

Embodiment 3

[0081]Example 3 Proliferation of Aedes albopictus densovirus-6 in the body of Aedes albopictus and breeding water

[0082] Materials and methods: TaKaRa miniBEST Viral RNA / DNA Extraction Kit and SuperReal PreMix Plus used in this experiment were purchased from Beijing Tiangen Biotechnology Co., Ltd.

[0083] Take about 1,000 first-instar larvae of Aedes albopictus that have just cast off their skin and divide them into groups of 200 for a total of five groups, adding to 2ml 10 10 AalDV-6 virus at a concentration of 1 / mL is used to detect the virus concentration in Aedes albopictus larvae and breeding water on the 1st, 3rd, 6th, 9th, and 12th days after infection. After 24 hours, add dechlorinated water to 200 mL for normal feeding. At the same time, one group was taken out, and 200 microliters of water was collected for detection of the virus concentration in the breeding water. All larvae were ground with 1mL PBS buffer, centrifuged at 3750 rpm for 15 minutes, the supernata...

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Abstract

The invention discloses an aedes albopictus densovirus, the name of which is aedes albopictus densovirus AalDV-6 which is preserved at CCTCC with the preservation number of CCTCC NO. V201652. The invention also discloses an application of the aedes albopictus densovirus in preparing a biological mosquitocide.

Description

technical field [0001] The invention belongs to the technical field of mosquito densovirus, and in particular relates to Aedes albopictus densovirus and application thereof. Background technique [0002] Mosquito-borne infectious diseases are diseases transmitted by mosquitoes, including malaria, filariasis, dengue fever, Japanese encephalitis, and yellow fever. Mosquito-borne diseases are widespread, highly transmissible, and highly morbid, and despite centuries of efforts to control them, mosquito-borne diseases continue to flourish around the world. Hundreds of millions of people are infected with malaria every year in the world, and malaria kills more than 1 million children every year, mainly in sub-Saharan Africa. The main vectors of dengue fever are Aedes albopictus and Aedes aegypti. In the past few decades, with the global spread of its vectors, the scope of transmission has gradually expanded. eradicated areas and cause widespread hemorrhagic fever, while Aedes a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A01N63/40A01P7/04C12R1/93
CPCA01N63/00C12N7/00C12N2750/14021C12N2750/14032C12N2750/14051
Inventor 顾金保刘培文陈晓光
Owner 陈晓光
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