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Long-term storage composite lactobacillus powder for aquaculture and preparation method thereof

A technology for aquaculture and long-term preservation, applied in the direction of lactobacillus, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of difficult to further increase the bacterial content and short shelf life, so as to alleviate or prevent the occurrence of diseases, reduce the Vibrio content in water and the effect of promoting the growth of prawns

Inactive Publication Date: 2017-05-31
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional carbon and nitrogen source optimization method can make Lactobacillus reach 10 9 After CFU / ml, it is difficult to further increase the content of bacteria after increasing the content of carbon and nitrogen sources
Commercially available Lactococcus maintains longer viability, while Lactobacillus products have a shorter shelf life, but Bacillus has special physiological activity different from cocci

Method used

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  • Long-term storage composite lactobacillus powder for aquaculture and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 A kind of preparation of probiotics

[0041] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservation number CCTCC NO: M2013293 and Lactobacillus acidophilus (Lactobacillus acidophilus) PL7 preservation number CCTCC NO: M2013294 Cultivation: the starting strains are cultivated in the order of slant, liquid seed, and liquid fermentation , the slant and seed medium are conventional MRS medium: fermentation medium formula (W / V): molasses 6%, brown sugar 3%, yeast extract 0.03%, peptone 0.6%, oligosaccharides 0.5%, magnesium sulfate 0.05%, Manganese sulfate 0.05%, calcium carbonate 0.5%, fermentation condition is 37 ℃, static culture for 36-48h, the number of viable bacteria ≥ 10 9 CFU / mL;

[0042] The Lactobacillus powder obtained by freeze-drying after centrifugation is mixed evenly with 1% vitamin C, 20% by weight of glucose, and equal times of weight of starch. Water-soluble edible gum, cyclodextrin, cellulose...

Embodiment 2

[0046] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0047] 1. Materials and methods

[0048] 1. Test product: the compound probiotics of Example 1.

[0049] 2. Experimental prawns: the prawns were divided into 6 groups including two groups of control groups, 300 small prawns with a body length of 2 cm in each group.

[0050] 3. Test water body: The test water is taken from sand-filtered seawater.

[0051] 4. Test site: The test is carried out in a glass fiber tank of 80cm×100cm×100cm (length×width×height). The test cylinder is placed indoors under natural conditions near the window.

[0052] 5. Test method: The test period is 60 days. The test adopts the conventional prawn feed mixing material feeding. The test group mixes the material once a day and feeds it. The mixing material amount is 10% of the feeding amount, and the control group is only fed under the same feeding amount. On the 30th day, 45th day, and 60th day of the te...

Embodiment 3

[0060] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3

[0061] 1. Test product: the compound probiotics of Example 1.

[0062] 2. Experimental prawns: The prawns from seedlings to 30 days old are used throughout.

[0063] 3. Test site: There are 4 experimental ponds and 4 control ponds in a prawn farm, with a volume of 4m×4m×1.5m and a water depth of 1m.

[0064] 4, test method: during daily raising, get embodiment 1 compound bacterial agent mixing material and throw something and feed. The material was mixed twice a day, and the dosage of compound bacteria was 10% of the feed amount fed each time. And the control group prawns were fed the feed without bacteria agent.

[0065] 5. Test results:

[0066] The use effect of the composite microbial agent of embodiment 1 is mainly reflected in aspects such as controlling the number of vibrio in the water body and preventing and treating prawn di...

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Abstract

The invention discloses long-term storage composite lactobacillus powder for aquaculture and a preparation method thereof. The composite lactobacillus powder contains lactobacillus plantarum, lactobacillus acidophilus and micro-capsule extine. The preparation method comprises the following steps: activating the strain and scribing on a corresponding panel, and inoculating the activated monoclonal product into a seed culture medium for culture; with a fermentation culture medium as a first-level seed culture medium, adding either fructo-oligosaccharide or trehalose as a lactic acid bacterium growth promoting agent, and sterilizing; inoculating the cultured primary activated seeds into the first-level seed culture medium for culture, and inoculating into the fermentation culture medium for culture; after the fermentation, centrifugally collecting thalli by a tubular centrifuge to obtain bacterial sludge; mixing uniformly and spreading on a sample plate of a sample freeze dryer, and dividing the bacterial sludge into small pieces; mixing the obtained lactobacillus plantarum powder with vitamin C, glucose and starch; and emulsifying, mixing, granulating and drying the sample to obtain the long-term storage composite lactobacillus powder for aquaculture with embedded lactic acid bacterium thalli.

Description

technical field [0001] The invention relates to aquaculture, in particular to a long-term preservation compound lactobacillus powder for aquaculture and a preparation method thereof. Background technique [0002] The application of antibiotics has produced huge economic benefits in animal husbandry. However, the negative effects associated with the extensive use of antibiotics are becoming more and more prominent, mainly manifested in the emergence of drug resistance of pathogenic microorganisms and drug residues in animals, thus causing direct or potential harm to human health. The application of antibiotics in feed encounters severe challenges. And at present, the method of improving the quality of meat protein in my country's feed gradually arouses people's worries, such as the addition of prohibited drugs such as Sudan red and clenbuterol, and the excessive addition of antibiotics to improve the quality of meat and eggs, etc., which pose a threat to food safety. It is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/80A23K10/18A23K20/163A23K20/147A61K35/747A61P31/04C12R1/25C12R1/23
CPCA61K35/747C12N1/20A23V2400/113A23V2400/169A61K2300/00
Inventor 邵宗泽单大鹏李旭林剑辉王丽萍
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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