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A Star Arrowhead strain scsio 43502 and its application

A technology of arrowhead bacteria and nitrogen-fixing bacteria fertilizer, which is applied in the field of microorganisms to achieve good nitrogen-fixing activity and the effect of promoting the growth of seaweed

Active Publication Date: 2020-03-24
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrogen provided by biological nitrogen fixation is used to supply seagrass plants to grow, and the formation can effectively promote the restoration of island and reef ecosystems. There are many studies on the production and research of nitrogen-fixing bacterial fertilizers in other ecosystems, but have not yet been reported.

Method used

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  • A Star Arrowhead strain scsio 43502 and its application
  • A Star Arrowhead strain scsio 43502 and its application
  • A Star Arrowhead strain scsio 43502 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: Isolation, purification and identification of Sagittula stellata SCSIO 43502

[0020] 1. Isolation and purification of Sagittula stellata SCSIO 43502

[0021] The sample was collected in June 2015. The bacterium SCSIO 43502 was isolated and purified from the rhizosphere of the seagrass halophaga in the coral island and reef ecosystem of Sansha City, Hainan Province, China.

[0022] 2. Identification of Sagittula stellata SCSIO 43502

[0023] 1) Morphological identification: under an optical microscope, the bacteria SCSIO 43502 in the logarithmic growth phase were picked and cultured, placed on a glass slide, fixed with a cover glass, and observed. The bacterium SCSIO 43502 obtained in this example is a straight rod-shaped, Gram-negative bacterium, does not form spores, and is easy to form rosettes and aggregations in the stationary phase, round, with raised edges, and the color is light cream .

[0024] 2) Determination of bacterial nitrogen fixation activ...

Embodiment 2

[0031] Example 2: 16SRNA and nitrogen fixation gene amplification of bacterial strains

[0032] Select the pure culture of bacteria SCSIO 43502 obtained by separation and purification in Example 1 to carry out DNA extraction and purification. For the extraction and purification of DNA in this embodiment, its operation method refers to Dong Xiuzhu's "Common Bacteria System Identification Manual" P 409 . Using bacterial 16S 27F / 1492R (Weisenburg et al., 1991, 16S ribosomal DNA amplification forphylogenetic study., Journal of bacteriology, 1991 173 (2): 697-703 and nifH gene universal primer PolF / PolR (refer to Poly et al. , 2001, Comparison of nifH Gene Pools in Soils and Soil Microenvironments with Contrasting Properties, Applied and Environmental Microbiology, 2001, 67 (5): 2255-2262) PCR amplification was carried out respectively, and the PCR reaction system is shown in Table 1.

[0033] Table 1 PCR reaction system

[0034]

[0035] The PCR reaction conditions were: pre-...

Embodiment 3

[0036] Example 3: Effectiveness test of bacterial fermentation broth on the growth-promoting effect of seaweed halophaga

[0037] Use the selective liquid nitrogen-free liquid to improve the medium (the formula is the same as in Example 1), shake the S. stellata SCSIO 43502 bacterial liquid after overnight fermentation at 28 ° C, and after the strain grows fully, use ultraviolet spectrophotometry Measuring the OD (optical density) value of each bacterial strain suspension, when OD 660 When the value is 0.6, take 20mL and add them into the seaweed rhizosphere (experimental group) in the seagrass philodendron culture tank respectively, and only add 20mL ultrapure water (CK group) in the control group, each three repetitions, and then place them in the same culture Under the conditions, the light-dark cycle is 12h:12h (light: dark cycle), and the light intensity is 200μE / m 2 / s, cultivated at 30°C, after 45 days, count the number of roots and measure the length of the roots, the...

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Abstract

The invention discloses a star arrow fungus SCSIO 43502 and its application. Sagittula stellata SCSIO 43502, deposit number: CCTCC NO: M 2016504. The present invention isolates and screens a strain of Sagittula stellata SCSIO 43502 from the rhizosphere of the seagrass halophaga in the coral island reef ecosystem. Therefore, the Sagittula stellata SCSIO 43502 has high value and broad application prospects in the preparation of marine microbial fertilizers and in the protection and restoration of island and reef ecology.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and in particular relates to a star arrowhead strain SCSIO 43502 and its application in the preparation of growth-promoting nitrogen-fixing bacterial fertilizer. Background technique: [0002] Coral island reefs are a special ecosystem developed by the accumulation of calcium carbonate skeletons and other biological debris of reef-building corals. They have extremely high productivity and biodiversity, so they are known as "tropical rainforests in the ocean" and "Oasis in the Blue Desert". However, due to the impact of global climate change and human activities, the world is in a state of decline. It is estimated that about 10% of the world's coral reefs have been severely damaged irreversibly, and about 50% of the coral reefs are on the verge of collapse, among which Southeast Asia The degradation of corals is even more serious. About 95% of corals in this region are under threat. If the decl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/205C12R2001/01
Inventor 凌娟董俊德张燕英周卫国王聪林丽云曾思泉杨清松
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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