Method of extracting stevioside from stevia rebaudiana bertoni by simulated moving bed chromatography (SMBC) method

Abstract

The invention provides a method of extracting stevioside from stevia rebaudiana bertoni by a simulated moving bed chromatography (SMBC) method and belongs to purification methods of effective components from natural plants. In the technology of extracting the stevioside, purification with column chromatography is a key step, but resin is large in dosage, low in utilization ratio and long in regeneration period, can produce a great quantity of waste water and is of intermittent type operation mostly. According to the method, an SMBC separation technology with C18 as a stationary phase can steadily, continuously, automatically and efficiently extract the stevioside from a crude stevioside extract on a large scale, and the stationary phase and a mobile phase can be repeatedly utilized, so that the purification cost is lowered, and the SMBC separation technology belongs to an environmental-friendly separation process; and in combination with a water infiltration leaching method and an impurity removal method by a chromatography method, the water consumption and the energy consumption are lowered, flocculation impurity removal is not needed, the stationary phase does not need to be specially regenerated, the waste water drainage is reduced, and the technology is simple.

Description

Technical field [0001] The invention relates to a method for purifying effective components of natural plants, in particular to a method for extracting steviol glycosides from stevia by simulated moving bed chromatography. Background technique [0002] The steviol glycoside in stevia is known as the "best natural sweetener". It has high sweetness, low calories, non-toxic and stable. Many countries at home and abroad have widely used steviol glycosides in food, medicine, health care, and beverages. And other industries. Rebaudioside A (RA) is the monomer with the highest sweetness and best taste among steviol glycosides. At present, the general process for extracting steviol glycosides from stevia includes: water immersion extraction, coarse filtration, flocculant removal, fine filtration, column chromatography purification, concentration drying and crystallization. In these processes, water immersion extraction consumes a lot of water, requires distilled water to concentrate, a...

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Problems solved by technology

In these processes, the water consumption is large during water soaking and extraction, which requires distilled water concentration and high energy consumption; when the flocculant is used to remove impurities, a large amount of precipitation will be generated after adding flocculant to the leachate, although most impurities can be removed, but it will also be adsorbed. A part of steviol glycosides reduces the product yield and produces a large amount of salt at the same time; column chromatography purification is a key step of the process, in addition to removing a small amount of pigment, tannin and other sugars remaining in the stevioside extract, it can also desalt and other impurities , but the amount of resin and mobile phase is large, the utilization rate of resin is low, and the resin needs to be regenerated. The regeneration cycle of desalination and decolorization resin is generally very long, and a large amount of waste water is generated