Nitroreductase-specific fluorescent probe and preparation thereof and application in tumor-targeted fluorescence imaging and monitoring of tumor hypoxia

Abstract

The invention provides a nitroreductase-specific fluorescent probe and the preparation thereof and a reagent for applying the nitroreductase-specific fluorescent probe to tumor-targeted fluorescence imaging and monitoring of tumor hypoxia. The reagent is connected by tumor biomarker identifying groups (sensor), tumor targeted groups (target) and fluorescent groups (dye) via chemical bonds. The reagent can be used in the tumors with hypoxic microenvironment and high expression of nitroreductase based on tumor hypoxia and the high expression of nitroreductase in hypoxic tumors. Combined with a fluorescence imager, the reagent can be used in tumor targeted fluorescence imaging, tumor hypoxia monitoring and the monitoring of tumor metastasis, etc. The reagent realizes high sensitivity and high specificity in application and provides an effective tool for medical study of cancer and clinical monitoring and treatment of tumor metastasis, thereby having a good application prospect in tumor-targeted fluorescence imaging, tumor hypoxia monitoring and monitoring of tumor metastasis.

Description

technical field [0001] The invention belongs to the field of biomedical technology, and relates to a class of detection reagents and their preparation methods and applications, specifically specific fluorescent probes for nitroreductase and their preparation methods, and are used for tumor-targeted fluorescence imaging and monitoring of tumor hypoxia Applications. Background technique [0002] The growth and proliferation of cells depend on sufficient oxygen and energy supply. In tissues with normal oxygen supply, about 90% of the energy source of cells depends on the aerobic oxidation of mitochondria. However, in the tumor tissue, the uncontrolled growth and proliferation of tumor cells consume a large amount of nutrients and oxygen, and the new blood vessel network cannot be established in a timely and effective manner, or the structure and function of the new blood vessel network are abnormal, and there is a temporary closure or "blind end". "; and the permeability is hi...

AI Technical Summary

Problems solved by technology

However, in the tumor tissue, the uncontrolled growth and proliferation of tumor cells consume a large amount of nutrients and oxygen, and the new blood vessel network cannot be established in a timely and effective manner, or the structure and function of the new blood vessel network are abnormal, and there is a temporary closure or "blind end". ”; and the permeability is high, and the extravasation of fluid into the interstitial space leads to an increase in the viscous resistance of blood flow

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