Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanometer chitosan ultrasonic microbubble targeting prostate cancer cells and preparation and application thereof

A nano-chitosan, ultrasonic microbubble technology, applied in echo/ultrasonic imaging agents, preparations for in vivo tests, medical preparations with non-active ingredients, etc., can solve the problem of low tissue penetration, ultrasonic microbubble targets Low tropism and other problems, to achieve the effect of strong tissue penetration, high tissue targeting, and strong penetration

Active Publication Date: 2017-05-31
SOUTH CHINA UNIV OF TECH
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a targeted nano-chitosan with high targeting and strong tissue penetration for the shortcomings of existing ultrasonic microbubbles, such as low targeting and low tissue penetration. Targeted prostate nano-ultrasonic microbubbles

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanometer chitosan ultrasonic microbubble targeting prostate cancer cells and preparation and application thereof
  • Nanometer chitosan ultrasonic microbubble targeting prostate cancer cells and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) 40 parts by weight of chitosan, 20 parts by weight of methacryloyloxyethyltrimethylammonium chloride, and 20 parts by weight of 3-maleimidopropionic acid hydroxysuccinimide ester are mixed, 60 React at ℃ for 6 hours to obtain quaternized modified chitosan; add 30 parts by weight of prostate-specific antibody PSA antibody (Shanghai Ruiqi Technology Co., Ltd., model Rsm-1625M) to the modified chitosan, add Potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with pH=6.5, make the pH of the system=6.5, and react at 60°C for 4 hours to obtain the chitosan-prostate specific antibody complex;

[0039] 2) Mix 10 parts by weight of dodecafluoropentane, 5 parts by weight of chitosan-prostate specific antibody complex and 10 parts by weight of distilled water, and react for 1.5 hours to form colostrum;

[0040] 3) Add 10 parts by weight of Tween 60 to 40 parts by weight of castor oil and dropwise add 10 parts by weight of n-butanol as the organic phase...

Embodiment 2

[0044] 1) 50 parts by weight of chitosan, 30 parts by weight of methacryloyloxyethyltrimethylammonium chloride, and 20 parts by weight of 3-maleimidopropionic acid hydroxysuccinimide ester are mixed, 60 React at ℃ for 6 hours to obtain quaternized modified chitosan; add 30 parts by weight of prostate specific antibody PSA antibody (Shanghai Ruiqi Technology Co., Ltd., model is Rsm-1625M) to modified chitosan, add Potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with pH=6.5, make the pH of the system=6.5, and react at 60°C for 4 hours to obtain the chitosan-prostate specific antibody complex;

[0045] 2) Mix 40 parts by weight of nitrogen, 10 parts by weight of chitosan-prostate specific antibody complex and 20 parts by weight of distilled water, and react for 1.5 hours to form colostrum;

[0046] 3) Add 10 parts by weight of Tween 60 to 40 parts by weight of castor oil and dropwise add 10 parts by weight of n-butanol as the organic phase, stir evenly...

Embodiment 3

[0050] 1) 60 parts by weight of chitosan, 20 parts by weight of methacryloyloxyethyltrimethylammonium chloride, and 20 parts by weight of 3-maleimidopropionic acid hydroxysuccinimide ester are mixed, 60 Reaction under ℃ for 6 hours, obtains the modified chitosan of quaternization; In modified chitosan, add 30 weight parts prostate-specific antibody PSMA antibody (commercial drug reagent, Shanghai Rui Qi Technology Co., Ltd., model is Rsm- 1625M), adding potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with pH=6.5, making the pH of the system=6.5, and reacting at 60°C for 4 hours to obtain the chitosan-prostate specific antibody complex;

[0051] 2) Mix 40 parts by weight of octafluoropropane, 5 parts by weight of chitosan-prostate specific antibody complex and 25 parts by weight of distilled water, and react for 1.5 hours to form colostrum;

[0052] 3) Add 10 parts by weight of Tween 60 to 40 parts by weight of castor oil and dropwise add 10 parts ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of medical preparations, and discloses nanometer chitosan ultrasonic microbubble targeting prostate cancer cells and preparation and application thereof. A method comprises the following steps of (1) mixing chitosan, methacryloyloxyethyl trimethyl ammonium chloride and N-succinimidyl 3-maleimidopropionate, and heating to react, so as to obtain modified chitosan; adding a prostate antigen, adjusting the pH (potential of hydrogen) value of a system, and continuing to react, so as to obtain a complex; (2) stirring and mixing an inert matter with low boiling point, the complex and water, so as to obtain primary emulsion; (3) adding a dispersant and an additive into an oil-phase solvent, uniformly mixing, adding the primary emulsion, stirring at high speed, adding a crosslinking agent to cure, adding a precipitating agent to precipitate, washing, centrifuging at high speed, and drying particles, so as to obtain the ultrasonic microbubble. The nanometer chitosan ultrasonic microbubble has the advantages that the targeting property is good, the tissue penetration ability is strong, the affinity is high, and the nanometer chitosan ultrasonic microbubble is suitable for the ultrasonic developing of the prostate cancer cells.

Description

technical field [0001] The invention belongs to the field of medical preparations, and in particular relates to a nano-chitosan ultrasonic microbubble targeting prostate cancer cells, a preparation method and application thereof. Background technique [0002] Ultrasound contrast agents (UCAs) have been continuously developed since Gramiak's first discovery of tiny air bubbles that enhanced ultrasound imaging in 1967, and ultrasound detection techniques have evolved with them. With the development of ultrasound contrast agents, ultrasound contrast agents are no longer limited to ultrasound imaging, but have begun to develop towards drug delivery. Therefore, ultrasound molecular imaging technology based on ultrasound contrast agents combined with drug delivery systems has been developed. It is clinically used in the diagnosis, treatment and evaluation of certain diseases. [0003] At present, nano-microbubbles can enter the extravascular space and perform ultrasound imaging o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K49/22A61K9/51A61K47/36A61K47/42A61K47/04A61K47/06A61K47/20A61P35/00
CPCA61K9/5115A61K9/5123A61K9/5161A61K9/5169A61K49/223
Inventor 黄岳山邓惠良劳永华郭圣文黄育铭
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com