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Application of d-limonene in preparing drug for treating non-smoking lung cancer

A d-limonene, non-smoking technology is applied in the application field of d-limonene in the preparation of a drug for treating non-smoking lung cancer, and can solve the problems of high malignancy of small cell lung cancer, reduced patient survival rate, and difficulty in clinical promotion and application, etc. The effect of reducing cell invasion, reducing the number of tumors and improving the disease

Inactive Publication Date: 2017-05-31
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of the above drugs and the lack of uniform and standardized mutation detection, it is difficult to apply them clinically.
In addition, resistance to some newer regimens emerges early in treatment
Small cell lung cancer has a high degree of malignancy and poor prognosis, and postoperative intrathoracic recurrence is the main recurrence mode, which seriously reduces the survival rate of patients

Method used

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  • Application of d-limonene in preparing drug for treating non-smoking lung cancer
  • Application of d-limonene in preparing drug for treating non-smoking lung cancer
  • Application of d-limonene in preparing drug for treating non-smoking lung cancer

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Experimental program
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Effect test

Embodiment 1

[0026] In vitro research on MTT experiment: Guo Yi, Sophie Sun and others pointed out in the literature that adenocarcinoma is the most common type of non-smoking lung cancer, so this study uses A549, H1299 cell lines "digest logarithmic growth cells with 0.25% trypsin Make a cell suspension, inoculate the cells in a 96-well culture plate with a volume of 100 μL per well, and store at 37°C in 5% CO 2 incubator. After the cells adhered to the wall, 0mM, 0.5mM, and 1mM D-limonene were added. Each group has 6 replicate wells. After 24, 48, and 72 hours respectively, 50 μL of 1×MTT solution was added to the wells to be tested, and after incubation for 4 hours, the optical density (OD) value at a wavelength of 550 nm was measured with a microplate reader. figure 1 It was shown that the effect of D-limonene on the proliferation of A549 and H1299 lung cancer cells was detected by MTT method. The results showed that after 0.5mM and 1mM D-limonene treated cells for 24h, 48h, and 72h...

Embodiment 2

[0028] Cell scratch test: Take an appropriate amount of logarithmic phase cells (2×10 5 cells / mL), after being adhered to the wall, the degree of cell confluence reached 100% after 24 hours of culture, a straight line of equal width was drawn with a sterile 10 μL pipette tip, the exfoliated cells were washed with PBS, and the cells containing different concentrations of 0mM, 0.5mM and 1mM were added D-limonene medium. Place at 37°C 5% CO 2 Under the condition of culturing, the cell migration was observed under a 200× microscope, and the images of cell migration were taken at 0 and 24 hours. figure 2D-limonene inhibits the migration of lung cancer cells A549 and H1299. The results show that after 0.5mM and 1mM D-limonene treatment, the migration of non-smoking lung cancer cells A549 and H1299 is inhibited.

Embodiment 3

[0030] Apoptosis: Take an appropriate amount of logarithmic phase cells (2×10 5 cells / mL), after 24 hours of attachment to the wall, the cells were treated with 0mM, 1mM, and 1.5Mm D-limonene for 48 hours, and the apoptosis was detected with an apoptosis kit. image 3 The results showed that after 1mM and 1.5mM D-limonene treatment, the apoptosis of lung cancer cells A549 and H1299 increased.

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Abstract

The invention discloses an application of d-limonene in preparing a drug for treating a non-smoking lung cancer. The d-limonene can reduce a growth rate of a tumor in a nude mouse, reduces tumor formation quantity of the non-smoking lung cancer and therefore, has a certain improvement effect on the non-smoking lung cancer. In addition, the d-limonene can effectively inhibit proliferation of lung cancer cells, reduces metastasis and invasion ability of the non-smoking lung cancer cells and promotes cell apoptosis; the d-limonene is indicated to have the better improvement effect on the state of the non-smoking lung cancer; and a new selection is provided for clinical adjuvant treatment of the non-smoking lung cancer.

Description

technical field [0001] The invention belongs to the field of new application of medicines, and more specifically relates to the application of D-limonene in the preparation of medicines for treating non-smoking lung cancer. Background technique [0002] Lung cancer is currently the malignant tumor with the highest morbidity and mortality in the world. Compared with other cancers, its 5-year survival rate is only 15%. Classified according to the epidemiological characteristics of lung cancer, although previous studies have shown that smoking is a recognized risk factor associated with lung cancer, more and more studies have shown that the incidence of non-smoking lung cancer is showing an increasing trend year by year. In recent years, Guo Yi et al. have shown in the literature of "Research on Molecular Genetics of Non-smoking Lung Cancer" that non-smoking lung cancer (Lung cancer in the never smokers, LCINS) occupies the forefront among the causes of global cancer deaths. I...

Claims

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Application Information

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IPC IPC(8): A61K31/015A61P35/00
CPCA61K31/015
Inventor 钱碧云邓晓蓓冯楠楠张晓红叶晓飞李雪俞晓王玉林虹燕林玉兰钱颖张硕张维拓黄亨烨马荣钠
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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