Formula feed capable of improving intestinal barrier function and digestive enzyme activity of early-weaned piglets, and feeding method of formula feed
A technology of intestinal barrier function and digestive enzyme activity, applied in animal feed, animal feed, applications, etc., can solve problems such as inhibition of animal growth and development and impact on animal health
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Embodiment 1
[0033] Example 1 Effects of different treatments of soybean meal and Lactobacillus acidophilus on growth performance and intestinal morphology of weaned piglets
[0034] 1.1 Materials and methods
[0035] 1.1.1 Test material
[0036] The fermented soybean meal, expanded soybean, and Lactobacillus acidophilus culture were provided by Shanghai Yuanyao Biotechnology Co., Ltd. The crude protein content of the fermented soybean meal, expanded soybean, and Lactobacillus acidophilus culture were 50%, 35%, and 20% respectively.
[0037] 1.1.2 Experimental animals and group design
[0038] 240 21-day-old weaned piglets with similar genetic background, parity and body weight were selected and divided into 5 treatments according to random block design, which were control group A, test group B, test C group, test D group and test E Group, 4 replicates per treatment, 12 piglets per replicate. The test period is 21 days.
[0039] 1.1.3 Test diet
[0040] The diet formula was prepared a...
Embodiment 2
[0077] Example 2: Effects of different treatments of soybean meal and Lactobacillus acidophilus on protein metabolism, intestinal mucosal digestive enzyme activity and intestinal mucosal intestinal immunoglobulin
[0078] 2.1 Materials and methods
[0079] 2.1.1 Test material
[0080] Test material is the same as embodiment 1.
[0081] 2.1.2 Experimental animals and group design
[0082] Experimental animals and group design are the same as in Example 1.
[0083] 2.1.3 Test diet and feeding method
[0084] The basic diet composition and feeding method are the same as in Example 1.
[0085] 2.1.4 Feeding management
[0086] Feeding management and feeding methods are the same as in Example 1.
[0087] 2.1.5 Sample collection
[0088] A small amount of the prepared diet was randomly selected and mixed, sealed bags were used to sample 500g by quartering and stored in a -20°C refrigerator for the determination of fermented soybean meal, puffed soybean, puffed soybean meal, 3...
Embodiment 3
[0134] Example 3: Comparison of the effects of different feeding periods using different treatments of soybean meal and different contents of Lactobacillus acidophilus cultures on the growth performance and intestinal mucosal digestive enzyme activity of weaned piglets
[0135] 3.1 Materials and methods
[0136] 3.1.1 Test material
[0137] The expanded soybean and the culture of Lactobacillus acidophilus are provided by Shanghai Yuanyao Biotechnology Co., Ltd. The crude protein content of the expanded soybean and the culture of Lactobacillus acidophilus are 35% and 20% respectively.
[0138] 3.1.2 Experimental animals and group design
[0139] 216 21-day-old weaned piglets with similar genetic background, parity and body weight were selected and divided into 6 treatments according to the random block design, which were experimental group A, experimental group B, experimental group C, experimental group D, and experimental E Group, test group F, each treatment has 3 repetiti...
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