Preprocessing method for soil sample for detection of hydrocarbon oxidizing bacteria in oil and gas microbial exploration

A technology for microbial exploration and hydrocarbon oxidizing bacteria, applied to biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as high cost, difficult transportation, and difficulty in ensuring low temperature, and achieve high applicability

Active Publication Date: 2017-05-24
ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Freezing or cryopreservation must ensure that the sample requires a strict low temperature process from collection to transportation, and it is often difficult to ensure low temperature in field construction
[0006] 2) Difficulty in tran

Method used

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  • Preprocessing method for soil sample for detection of hydrocarbon oxidizing bacteria in oil and gas microbial exploration
  • Preprocessing method for soil sample for detection of hydrocarbon oxidizing bacteria in oil and gas microbial exploration
  • Preprocessing method for soil sample for detection of hydrocarbon oxidizing bacteria in oil and gas microbial exploration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The area involved is a high-altitude Gobi area on the Qinghai-Tibet Plateau, which is a permafrost zone with a little vegetation on the surface. To carry out oil and gas microbial exploration in this area, it is necessary to lay out survey lines or survey nets to collect surface soil. Conventional frozen soil pretreatment and transportation methods require the preparation of temperature-controlled equipment such as refrigerators and ice packs, which are time-consuming and difficult to transport, and it is difficult to achieve rapid freezing of samples in high-altitude areas.

[0047] The specific implementation steps of soil sample pretreatment of the present invention are as follows:

[0048] 1 According to the actual exploration situation, select the survey line that has been laid out in the work area, and collect 20 samples, the sample collection interval is 250m, and the collection depth is 20cm.

[0049] 2 Each sampling point was collected according to the convent...

Embodiment 2

[0053] 1 The working area involved is the same as in Example 1. According to the actual exploration situation, the laid out survey line was selected in the working area, and 8 samples were collected, the sample collection interval was 250m, and the collection depth was 20cm.

[0054] 2. Each sampling point was collected according to the conventional method, placed in a sealed plastic bag, and immediately frozen in the same way as in Example 1, and all samples were quickly transported back to the laboratory.

[0055] 3 In the laboratory, transfer the sample from the sealed plastic bag to a kraft paper bag, seal it and place it in a blast oven at 30°C for 8 hours of drying. Afterwards, the sample was divided into two parts, one was called a dried sample, and the subsequent detection of hydrocarbon oxidizing bacteria was carried out immediately; the other was stored at room temperature, and the detection of hydrocarbon oxidizing bacteria was carried out two weeks later.

[0056]...

Embodiment 3

[0059] The area involved is the eastern plain area of ​​the Songliao Basin, which belongs to the high-latitude cold area, and the surface is farmland, where rice is grown. To carry out oil and gas microbial exploration in this area, it is necessary to lay out survey lines or survey nets to collect surface soil. Conventional frozen soil pretreatment and transportation methods require the preparation of refrigerators, ice packs and other temperature-controlling equipment, which is time-consuming and difficult to transport.

[0060] In order to compare the influence of different drying times on the counting results of hydrocarbon oxidizing bacteria, the following experiments were carried out:

[0061] 1 According to the actual exploration situation, select known oil and gas fields in the working area, design a survey line passing through the oil and gas fields, and collect 16 samples, numbered 1-16, of which samples No. 5-10 are located above the oil field, and the sample collect...

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Abstract

The invention relates to the technical field of microbial detection, and provides a preprocessing method for a soil sample for detection of hydrocarbon oxidizing bacteria in oil and gas microbial exploration, wherein the method comprises the following steps: 1) placing field-collected soil into an opening breathable container, and sealing an opening of the container; and 2) carrying out constant-temperature air blasting of the breathable container sealed in the step 1), to make the soil in the container dry. A test result shows that the soil after being pretreated by the method is used for detection of the hydrocarbon oxidizing bacteria, and the result is similar to that of a conventional freezing method. The soil after drying is placed at room temperature for two weeks and then is detected, and a result is similar to that of a freshly dried sample. The results show that the method is an alternative method for conventional cryopreservation, and has high applicability in study of the hydrocarbon oxidizing bacteria in oil and gas microbial exploration.

Description

technical field [0001] The invention relates to the technical field of soil microbial detection, in particular to a pretreatment method for soil samples used for the detection of hydrocarbon oxidizing bacteria in oil and gas microbial exploration. Background technique [0002] At present, the sample preservation method for environmental soil microbial detection is mainly to quickly freeze at low temperature after on-site collection, or keep it at 1-5°C for storage, that is, take a certain amount of soil samples in the field and seal them, then use refrigerators, liquid nitrogen, and ice packs to store them. Or use dry ice or other methods to freeze or keep at 1-5°C, and the whole process of transportation also needs to be kept in a frozen or low temperature state. Using preserved soil samples to detect microorganisms, the obtained microbial information is of great significance to fields such as agriculture, environmental protection and oil and gas exploration. [0003] Amon...

Claims

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Application Information

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IPC IPC(8): C12Q1/64
CPCC12Q1/64
Inventor 郝纯梅海
Owner ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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