Kit for rapidly extracting nucleic acid in amniotic fluid and chorionic tissue sample

Abstract

The invention discloses a kit for rapidly extracting a nucleic acid in an amniotic fluid and chorionic tissue sample. The kit is prepared from the following components: a magnetic bead pyrolysis binding solution, magnetic beads, a magnetic bead cleaning solution 1, a magnetic bead cleaning solution 2 and a magnetic bead eluent. A 96-hole pre-subpackaging deep-hole plate mode is adopted, and an automatic nucleic acid extractor can be matched to automatically extract the nucleic acid. A TCEP additive is added into the magnetic bead pyrolysis binding solution, so that the nucleic acid extraction efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid nucleic acid extraction kit in amniotic fluid and villi samples. The invention also relates to a method for extracting nucleic acid in amniotic fluid and villi samples by using the magnetic bead method. Background technique [0002] The basic unit of genetic characteristics of living organisms is nucleic acid, which is inseparable from the detection of nucleic acid in many fields such as genetic research, and the key premise is to extract effective and reliable nucleic acid, which is also the key in many nucleic acid molecular biology experiments. The key, such as qualitative PCR, molecular hybridization, real-time fluorescent quantitative PCR and other technologies. Nucleic acid plays an irreplaceable role in the whole molecular biology. [0003] In the prenatal diagnosis of pregnant women, nucleic acid testing of amniotic fluid and chorionic villi samples is very importan...

AI Technical Summary

Problems solved by technology

Although the column extraction method and the magnetic bead method do not use toxic and harmful substances such as phenol and chloroform in the process of extracting nucleic acids, they need to repeatedly open the cap to add liquid, discard liquid, and centrifuge steps, and frequently replace centrifuge tubes and pipette tips. The column extraction method requires continuous centrifugation, and the magnetic bead method must be careful not to remove the magnetic beads when discarding the liquid. When the number of samples is large, the entire step will be lengthy and complicated, resulting in low extraction efficiency, error-prone, and difficult to avoid cross-contamination

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