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Method for simultaneously producing vitamin K2 and nattokinase through Bacillus natto fermentation

A technology of Bacillus natto and nattokinase, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high cost and low yield, and achieve the improvement of co-production rate and low fermentation cost , Conducive to the effect of industrialization

Active Publication Date: 2017-05-24
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for co-producing vitamin K by fermentation of Bacillus natto 2 and nattokinase method, using optimized co-production medium to make Bacillus natto co-produce vitamin K 2 The output of nattokinase and nattokinase reached a relatively high level, which preliminarily solved the problem of vitamin K 2 And the problem of high cost and low yield in the fermentation process of nattokinase, and improved the utilization rate of raw materials to promote the process of industrialization

Method used

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  • Method for simultaneously producing vitamin K2 and nattokinase through Bacillus natto fermentation
  • Method for simultaneously producing vitamin K2 and nattokinase through Bacillus natto fermentation
  • Method for simultaneously producing vitamin K2 and nattokinase through Bacillus natto fermentation

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Embodiment 1

[0027] A method of co-producing vitamin K by fermentation of Bacillus natto 2 and nattokinase method, using Bacillus natto as a fermentation strain, optimizing the medium formulation, including strain activation, preparing seeds, inoculating fermentation medium, controlling the growth of Bacillus natto and the metabolite vitamin K in the fermentation process 2 and nattokinase production, the vitamin K in the fermentation broth 2 Separated from nattokinase.

[0028] Include the following steps:

[0029] (1) Strain activation: Streak Bacillus natto onto plate medium (recipe: peptone 5g / L, beef extract 5g / L, sodium chloride 3g / L, agar powder 20g / L, sterilized at 121°C 20min, place it at 30°C for cultivation, and then take a single colony and streak it to the test tube culture medium (the formula is: peptone 5g / L, beef extract 5g / L, sodium chloride 3g / L, agar powder 20g / L, Sterilized at 121°C for 20 minutes, placed at 30°C for use) for cultivation, wherein the culture condition...

Embodiment 2

[0040] A method of co-producing vitamin K by fermentation of Bacillus natto 2 and nattokinase method, using Bacillus natto as a fermentation strain, optimizing the medium formulation, including strain activation, preparing seeds, inoculating fermentation medium, controlling the growth of Bacillus natto and the metabolite vitamin K in the fermentation process 2 and nattokinase production, the vitamin K in the fermentation broth 2 Separated from nattokinase.

[0041] Include the following steps:

[0042] (1) Strain activation: Streak Bacillus natto onto plate medium (formulation: peptone 5-7g / L, beef extract 7g / L, sodium chloride 4g / L, agar powder 20g / L, 121°C Sterilize for 20 minutes, place it at 40°C for cultivation, and then take a single colony and streak it to the test tube medium (the formula is: peptone 7g / L, beef extract 7g / L, sodium chloride 4g / L, agar powder 20g / L L, sterilized at 121° C. for 20 minutes, placed at 40° C. for use) for cultivation, wherein the culture...

Embodiment 3

[0053] A method of co-producing vitamin K by fermentation of Bacillus natto 2 and nattokinase method, using Bacillus natto as a fermentation strain, optimizing the medium formulation, including strain activation, preparing seeds, inoculating fermentation medium, controlling the growth of Bacillus natto and the metabolite vitamin K in the fermentation process 2 and nattokinase production, the vitamin K in the fermentation broth 2 Separated from nattokinase.

[0054] Include the following steps:

[0055] (1) Strain activation: Streak Bacillus natto onto the plate medium (the formula is: peptone 6g / L, beef extract 6g / L, sodium chloride 3.5g / L, agar powder 20g / L, sterilized at 121°C Bacteria for 20 minutes, placed in 35 ° C for use), and then took a single colony and streaked it to the test tube medium (formulation: peptone 6g / L, beef extract 6g / L, sodium chloride 3.5g / L, agar powder 20g / L L, sterilized at 121° C. for 20 minutes, placed at 35° C. for use) for cultivation, where...

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Abstract

The invention discloses a method for simultaneously producing vitamin K2 and nattokinase through Bacillus natto fermentation. The method comprises the following steps: strain activation: performing streak culture on Bacillus natto in a plating culture medium, and culturing a single colony in a test tube culture medium; seed preparation: inoculating the Bacillus natto in the test tube into a seed culture medium, and culturing; production of nattokinase and vitamin K2: preparing fermentation culture medium for simultaneous production, taking the prepared seed solution after the fermentation culture medium for simultaneous production is cooled, inoculating, and producing the nattokinase and vitamin K2 under optimal culture conditions for simultaneous production; and separation and extraction of the nattokinase and vitamin K2: centrifuging the fermentation solution, separating the supernatant from the precipitate, adding inorganic high polymer flocculant into the supernatant, and taking chitosan as coagulant aid to separate the vitamin K2 and nattokinase in the supernatant. The method has the advantages of improving the raw material utilization ratio and being suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to the joint production of vitamin K by fermentation of bacillus natto 2 and nattokinase methods. Background technique [0002] Bacillus natto is a subspecies of Bacillus subtilis, a variety of physiologically active substances can be obtained after fermentation, vitamin K 2 , nattokinase, biological surfactants, biological polysaccharides, etc. Vitamin K 2 (menaquinone, abbreviated as MK, menaquinones), is a naphthoquinone derivative with biological activity of phylloquinone, a fat-soluble vitamin. According to the length of the isoprene side chain on C-3, there are 14 types of MK, usually represented by MK-n, where n refers to the number of isoprene units on the side chain. MK-4, MK-7, MK-9 biological activities are significantly higher than vitamin K 1 . Vitamin K 2 It can treat and prevent osteoporosis, prevent liver cirrhosis from progressing to liver canc...

Claims

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Application Information

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IPC IPC(8): C12N9/54C12P7/66C12R1/07
CPCC12N9/54C12P7/66C12Y304/21062
Inventor 郑之明王鹏孙孝娟刘会赵根海王丽王晗尉鸿飞吴荷芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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