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A kind of high-efficiency extraction method of proanthocyanidin components in thinned and young apples

A technology of proanthocyanidins and extraction method, which is applied in the direction of organic chemistry, can solve the problems of complex operation process and low extraction efficiency, and achieve the effect of efficient recycling and far-reaching economic and social benefits

Active Publication Date: 2019-04-09
PENGLAI MARINE SHANDONG +1
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Problems solved by technology

The operation process is complicated, the extraction rate is as high as 80%, and the extraction efficiency is low

Method used

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  • A kind of high-efficiency extraction method of proanthocyanidin components in thinned and young apples
  • A kind of high-efficiency extraction method of proanthocyanidin components in thinned and young apples
  • A kind of high-efficiency extraction method of proanthocyanidin components in thinned and young apples

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Embodiment Construction

[0009] A method for efficiently extracting proanthocyanidin components from thinned young apples, comprising the following steps:

[0010] 1) Preparation of the extract: take the cleaned apples and remove young fruits as raw materials, add water to make a pulp, add compound plant hydrolytic enzyme and cellulase to the pulp for double-enzyme hydrolysis, and obtain the product after double-enzyme hydrolysis The enzymatic solution is subjected to alcohol precipitation to obtain the crude extract of proanthocyanidins;

[0011] 2) Preparation of analysis solution: Concentrate the crude proanthocyanidin extract obtained in step 1) until it has no alcohol smell, add 8 to 20 times the weight of the crude extract and dilute it with water, then add gelatin to make the gelatin concentration reach 3 to 8 mg / mL for complexation React for 4 to 8 hours, filter after the reaction, collect the precipitate, stir and analyze the precipitate with water as the analysis solution, centrifuge, collec...

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Abstract

The invention relates to a method for efficiently extracting procyanidine ingredient from young apple fruits removed by thinning. According to the method, the procyanidine is extracted from a raw material (the young apple fruits removed by thinning) by virtue of combining a dual-enzymolysis technology and an albumen precipitation process with LH-20 gel column chromatography. The method is simple in technical process and high in extraction rate which reaches up to 90% or above, and reserves the functional active ingredients of the procyanidine to the greatest extent; the extracted procyanidine is high quality, has favorable activity on eliminating in-vivo free radicals and restraining proliferation of tumor cells, and can be directly used as a prescribed preparation or can be compounded with other functional ingredients for use. The method is capable of realizing high-value utilization of young apple fruits, and widening the excavation scope of apple resources; the extraction process is simple in operation and short in period; the prepared active ingredient is good in chemical stability, high in activity, free from toxic or side effects and suitable for large-scale production.

Description

technical field [0001] The invention relates to a high-value utilization of thinned apples, in particular to a method for efficiently extracting proanthocyanidins from thinned young apples, and belongs to the technical field of functional food processing. Background technique [0002] Apple, as the fruit of the apple genus, is very popular among consumers because of its rich functionality and nutrition, which is beneficial to eating and health care. In the annual apple production process, fruit thinning is a measure that must be taken to improve fruit quality in the apple production process. my country is the largest apple producing country in the world, and the number of young fruits produced by fruit thinning is large. Apple thinning fruit also has rich nutritional value, and its rich polysaccharides, polyphenols, vitamins, minerals and dietary fiber have become the basis for studying the functional characteristics of apple thinning fruit. At present, the thinly fallen ap...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 张轩铭韩利文侯海荣张姗姗李晓彬刘可春陈玉峰于涛孙博隋晓明吕宪峰魏新赛隋莎莎
Owner PENGLAI MARINE SHANDONG
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