Method for preparing microbe bacteria liquid for treating black-odor riverway
A technology of microbial bacteria liquid and river channel, applied in chemical instruments and methods, biological water/sewage treatment, bacteria, etc., can solve the problems of shrinking water area, low self-purification ability of water body, low feasibility of full coverage of rain and sewage diversion pipe network, etc. , to achieve the effects of stable growth, elimination of black odor in river channels, and rapid improvement of water quality
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Embodiment 1
[0017] Embodiment 1, a kind of microbial bacterium liquid preparation method of controlling black and smelly river course is characterized in that, comprises the following steps:
[0018] (1) Collect river sediments and analyze the diversity of sediment microbial communities through high-throughput sequencing experiments. The high-throughput sequencing process includes sediment DNA extraction, PCR amplification and product purification, and Miseq sequencing;
[0019] (2) Initial screening, take 5~10g of river sediment, inoculate in sterile denitrification bacteria enrichment culture solution, place it in a 30°C incubator for static culture, cultivate for 3~4 days, and insert fresh enrichment culture In the culture medium, expand the culture three times to obtain the preliminary screening of the dominant flora, detect the content of ammonia nitrogen and nitrate nitrogen in the culture medium, and take the mixed bacterial liquid with less nitrogen accumulation as the flora with d...
Embodiment 2
[0024] Embodiment 2, the preparation method of a kind of microbial bacterium liquid of controlling black and odorous river course described in embodiment 1, described nitrogen-removing bacteria enrichment medium is (NH4) SO4 (132) 1g, KNO3 (101) 2g, citric acid Sodium (C6H5Na3O7·2H2O, 294.07) 6g (C / N>5), K2HPO4 1g, magnesium sulfate heptahydrate 0.2g, distilled water 1000ml, sterilize at 121°C for 20min.
Embodiment 3
[0025] Embodiment 3, a kind of microbial preparation that is used for river course water quality restoration described in embodiment 1, the pH value of described trace element solution is 8.
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