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Method for preparing high-stability microbial electrochemical sensor

A microbial electrochemistry and sensor technology, applied in scientific instruments, instruments, material analysis by electromagnetic means, etc., can solve problems such as the limitation of the scope of application, and achieve the effect of fast detection and stable operation.

Active Publication Date: 2017-05-10
天津智慧大数据服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial electrochemical sensors are highly selective and can directly and quickly obtain the composition information of complex systems. However, they are easily affected by external environments such as organic solvents, strong acids, and temperatures. Therefore, conventional microbial electrochemical sensors evaluate the scope of application of complex systems. greatly restricted

Method used

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  • Method for preparing high-stability microbial electrochemical sensor
  • Method for preparing high-stability microbial electrochemical sensor
  • Method for preparing high-stability microbial electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A prepared highly stable microbial electrochemical sensor operates under strong acid conditions:

[0020] When realizing rapid and continuous water quality monitoring, the low pH value of the water body to be tested often limits the stable operation of the detection system, causing a large number of organisms in the sensor system to die or fall off, which directly affects the operation of the reactor and causes the monitoring system to be paralyzed.

[0021] The cell body of the sensor is composed of a plexiglass container with a diameter of 50mm and a height of 100mm and a cover made of polytetrafluoroethylene material, and the working electrode is inserted into the container at a distance of 0.25cm 2 The glassy carbon electrode, the reference electrode is 3.5M Ag / AgCl electrode and the counter electrode is 1cm 2 platinum sheet.

[0022] Add the effluent of the long-term acclimatized biophase-stabilized microbial fuel cell with nitrogen exposure for 10 min to the reac...

Embodiment 2

[0026] A prepared highly stable microbial electrochemical sensor operates at high temperature:

[0027] When realizing rapid and continuous water quality monitoring, when the external ambient temperature exceeds the maximum suitable temperature for organisms, it often limits the stable operation of the detection system, causing a large number of organisms in the sensor system to die or fall off, which in turn directly affects the operation of the reactor, resulting in monitoring The system is paralyzed.

[0028] The cell body of the sensor is made of polytetrafluoroethylene material with a diameter of 30mm and a height of 30mm, and the working electrode is inserted into the container at a distance of 0.1cm 2 The glassy carbon electrode, the reference electrode is 3.5M Ag / AgCl electrode and the counter electrode is 1cm 2 platinum sheet.

[0029] Add the effluent of the microbial fuel cell with long-term acclimatization and biophase stability to the reactor with nitrogen expos...

Embodiment 3

[0032] A prepared highly stable microbial electrochemical sensor operates at low temperature

[0033] When realizing rapid and continuous water quality monitoring, when the external ambient temperature is lower than the minimum suitable temperature for organisms, the stable operation of the detection system is often limited, causing a large number of organisms in the sensor system to die or fall off, which in turn directly affects the operation of the reactor, resulting in The monitoring system is paralyzed.

[0034] The cell body of the sensor is made of polytetrafluoroethylene material with a diameter of 100mm and a height of 200mm, and the working electrode is inserted into the container at a distance of 0.25cm 2 The glassy carbon electrode, the reference electrode is 3.5M Ag / AgCl electrode and the counter electrode is 1cm 2 platinum sheet.

[0035] Add the effluent of the microbial fuel cell with long-term acclimatization and biophase stability to the reactor for 30 minu...

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Abstract

The invention discloses a method for preparing a high-stability microbial electrochemical sensor. The microbial electrochemical sensor consists of an organic glass or polytetrafluoroethylene container with a volume of 20 to 1,570mL, a 0.1 to 10cm<2> glassy carbon or graphite working electrode, an Ag / AgCl or saturated calomel reference electrode, a 1cm<2> platinum sheet or platinum wire counter electrode and a weak alkaline solution of dopamine hydrochloride. The prepared microbial electrochemical sensor is used for a monitoring system for rapidly acquiring water body composition information in situ, and a monitoring result of the monitoring system in an extreme environment of a high-concentration organic solvent, a strong acid, high temperature and low temperature is true and effective. The method has the beneficial effects that the defect that a conventional microbial electrochemical system cannot run in the extreme environment of the strong acid, high temperature, low temperature, a high-concentration organic solution and the like is overcome by using the microbial electrochemical sensor, and a novel microbial electrochemical system is stable in running and rapid and accurate in detection.

Description

technical field [0001] The invention relates to the technical field of microbial electrochemistry, in particular to a preparation method of a highly stable microbial electrochemical sensor. Background technique [0002] Water quality safety is directly related to human health, therefore, rapid online monitoring of toxic substances in water is very important. Traditional water quality analysis techniques include chemical analysis, spectrophotometric analysis, atomic spectroscopy, chromatographic analysis, etc. However, due to the complexity of detecting water components and the characteristics of being greatly affected by the environment, it is difficult to meet the requirements of water quality testing, let alone Realize online detection. Microbial electrochemical sensors have attracted extensive attention due to their small size, low cost, good selectivity, high sensitivity, and fast response, enabling rapid on-line monitoring of complex systems. [0003] Microbial electr...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/416
CPCG01N27/3275G01N27/416
Inventor 王鑫杜青李田
Owner 天津智慧大数据服务有限公司
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