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Preparation of electrochemical biosensor device based on hemoglobin-nano-palladium-graphene composite materials and applied research of electrochemical biosensor device

A biosensor and hemoglobin technology, applied in the field of electrochemical biosensors and chemically modified electrodes, can solve problems affecting direct electrochemical behavior

Inactive Publication Date: 2017-05-10
HAINAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the electroactive centers of redox proteins are deeply buried in their structure, and it is generally difficult to directly transfer electrons with the substrate electrode, thus affecting their direct electrochemical behavior.

Method used

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  • Preparation of electrochemical biosensor device based on hemoglobin-nano-palladium-graphene composite materials and applied research of electrochemical biosensor device
  • Preparation of electrochemical biosensor device based on hemoglobin-nano-palladium-graphene composite materials and applied research of electrochemical biosensor device
  • Preparation of electrochemical biosensor device based on hemoglobin-nano-palladium-graphene composite materials and applied research of electrochemical biosensor device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Scanning Electron Microscopy Characterization of Pd-GR Nanocomposites

[0029] figure 1 Shown is a scanning electron microscope characterization of the Pd-GR nanocomposite at a magnification of 10000. The layered structure is the characteristic morphology of graphene, and palladium nanoparticles are deposited, intercalated, and embedded on the surface and inside of the layered graphene.

Embodiment 2

[0031] UV-Vis Absorption Spectroscopy Analysis

[0032] Ultraviolet-visible absorption spectroscopy is a common method for detecting whether the secondary structure of a protein changes. Whether the position of the Soret absorption band of the protein shifts can provide information on the physical structure of the protein. If the protein denatures or its physical structure changes, it will It will cause its absorption band to migrate or disappear. figure 2 The UV-vis absorption spectra of Hb aqueous solution and Pd-GR-Hb mixed solution are presented, the Soret absorption band of Hb in aqueous solution is at 405 nm (curve a) and the Soret absorption band of Hb-Pd-GR mixed solution is 405 nm ( Curve b) is exactly the same, indicating that Hb still maintains the original conformation after being mixed with Pd-GR composite material, and there is no structural change, which further shows that Hb still maintains the original shape in the composite film mixed with Pd-GR composite ma...

Embodiment 3

[0034] Infrared Spectroscopy Analysis

[0035] Fourier transform infrared spectroscopy (FT-IR) detects whether the secondary structure of the protein has changed by detecting the changes in the two absorption characteristic bands of amide I and amide II of the protein. The stretching vibration of the C=O bond contained in the peptide structure of the protein causes the amide I (1700-1600 cm -1 ) changes, N-H bond bending vibration and C-N bond stretching vibration caused by amide II (1620-1500 cm -1 ) changes. If the protein is changed or denatured, the two absorption bands of amide I and amide II may be shifted or even disappeared. Such as image 3 The infrared absorption bands of Hb amide I and amide II mixed with Pd-GR composites are shown at 1639 cm -1 and 1524 cm -1 (b), while the absorption band of native Hb is 1647 cm, respectively -1 and 1543 cm -1(a). From the similarity of the infrared spectrum shape, it can be considered that Hb basically maintains its origi...

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Abstract

The invention discloses preparation of an electrochemical biosensor device based on Hb-Pd-GR (hemoglobin-nano-palladium-graphene) composite materials and applied research of the electrochemical biosensor device. A preparation method of the electrochemical biosensor device includes the steps: taking ionic liquid 1-hexylpyridine hexafluorophosphate as modifying agents to prepare a CILE (carbon ionic liquid electrode); mixing Pd-GR and Hb, coating the surface of the electrode with mixture of the Hb-Pd and the GR, and drying the coated electrode; curing the dried electrode by the aid of a Nafion membrane to prepare a modified electrode Nafion / Hb-Pd-GR / CILE. Spectrum research shows that Hb in a composite membrane keeps that structures of the Hb cannot be denaturized, electrochemical behaviors of the Hb are researched by the aid of cyclic voltammetry, a pair of quasi-reversible reduction and oxidation peaks with good peak shapes are acquired in phosphate buffer solution, and pH (potential of hydrogen) of phosphate buffer solution is 3.0. The Pd-GR exists, so that electron transport rate between the Hb and a substrate electrode is accelerated, and the Hb can be directly electrochemically formed on the modified electrode. The Nafion / Hb-Pd-GR / CILE has good electro-catalytic performance for TCA (trichloroacetic acid) and NaNO2 (sodium nitrite) and can be applied to electrochemical sensing analysis of the TCA and the NaNO2.

Description

technical field [0001] The invention relates to the field of chemically modified electrodes in the fields of electrochemistry and electroanalytical chemistry, and the field of electrochemical biosensors. Background technique [0002] The development of chemically modified electrodes has made great progress in the aspects of preparation methods, composition and type of modifiers, electrode surface characterization and electrode process theory. Chemically modified electrodes break through the scope of traditional electrochemical methods limited to the study of the bare electrode / electrolyte interface, and initiate the study of artificially controlling the structure and function of the electrode surface from the chemical morphology. With the development of material science and technology, electrodes modified by various new materials have become a hot spot in electrochemical research, and electrodes modified by functionalized nanomaterials also show special functions and applica...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48B82Y30/00
CPCB82Y30/00G01N27/327G01N27/48
Inventor 孙伟陈玮牛学良文作瑞李晓燕赵文舒李小宝
Owner HAINAN NORMAL UNIVERSITY
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