Influenza virus reverse transcription loop-mediated isothermal amplification (RT-LAMP) kit and application thereof

Abstract

The invention discloses an influenza virus reverse transcription loop-mediated isothermal amplification (RT-LAMP) kit and application thereof. The kit comprises a RT-LAMP primer, 2* reaction buffer solution, enzyme mixture EM, a fluorescence visual detection reagent, ultrapure water and an influenza virus RNA template, wherein the RT-LAMP primer comprises outer primers F3 and B3, and inner primers FIP and BIP. Specificity detection, sensitivity detection and fluorescence visible detection prove that the RT-LAMP detection method can specifically detect influenza virus, can be used for monitoring reactions in real time and quantitatively detecting the number of influenza virus copies so as to quickly and correctly acquire the detection result, and brings convenience to simple, quick and reliable influenza virus detection.

Description

technical field [0001] The invention relates to the technical field of microbial detection, in particular to a rapid, visualized and real-time quantitative detection of influenza virus reverse transcription loop-mediated isothermal amplification kit and its application. Background technique [0002] Influenza viruses can cause infection and disease in many animals such as humans, poultry, pigs, horses, and bats, and are the pathogens of human and animal diseases such as human influenza, avian influenza, swine influenza, and equine influenza. Influenza virus is not strong against the outside world. Animal influenza viruses usually do not infect humans, and human influenza viruses generally do not infect animals, but pigs are an exception. Pigs can be infected with both human influenza viruses and avian influenza viruses, but they are mainly infected with swine influenza viruses. After a small number of animal influenza viruses adapt to humans, they can cause human influenza ...

AI Technical Summary

Problems solved by technology

The conventional method is the isolation and identification of viruses, and the results are accurate and reliable. However, the conventional identification method has the disadvantages of complex operation, complex equipment and long time, which is not conducive to the rapid diagnosis of influenza.

The method of molecular biology is to detect the specific gene of influenza virus by reverse transcription polymerase chain reaction (RT-PCR). Although the RT-PCR method is faster and more accurate than the conventional method, it requires expensive equipment and high cost. Agarose gel electrophoresis is required to determine the results, which is likely to cause laboratory contamination and lead to false positive results

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