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Salt-tolerant microbial agent as well as preparation method and application thereof

A microbial agent and salt-tolerant technology, applied in the field of environmental microorganisms, can solve problems such as limited application range and limited processing capacity, and achieve the effects of broadening the scope of application, improving composition, and strong impact resistance

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly relies on denitrification to remove nitrite to deal with COD, the treatment capacity is limited, and the scope of application is also limited to a certain extent.

Method used

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  • Salt-tolerant microbial agent as well as preparation method and application thereof
  • Salt-tolerant microbial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of embodiment 1 salt-tolerant microbial bacterial agent

[0023] (1) Strain activation: inoculate Paracoccus FSTB-2, Microbacterium kitami FSTB-4 and Pseudomonas stutzeri FSTB-5 on FSTB solid medium respectively, spread evenly and place it at a constant temperature of 35°C Activation in the incubator.

[0024] (2) Seed liquid culture: Use the inoculation loop to take the colonies of the three strains on the plate, and inoculate them in the corresponding FSTB liquid culture liquid, and culture them with shaking at 35°C and 150 rpm for 48 hours until logarithmic growth period, to obtain liquid bacterial agent seed solution.

[0025] (3) Scale-up cultivation: carry out scale-up cultivation of the above seed liquids in reactors with aeration devices, the COD concentration in the culture liquid is 2500mg / L, the salt content is 2.5wt%, the cultivation temperature is 35°C, and the dissolved oxygen is 2.5mg / L , and the culture time was 72 hours, thus ob...

Embodiment 2

[0029] Example 2 Microbial agent treatment of epichlorohydrin production wastewater

[0030] The epichlorohydrin production wastewater produced by a chemical plant has a salt content of 2.5wt%, a calcium ion content of 4000mg / L, and a COD of 1500mg / L. During the long-term operation of the system, the sludge performance has become calcified and the COD removal rate has been obvious. If the situation becomes worse, the effluent cannot meet the standard. The salt-tolerant microbial bacterial agent prepared in Example 1 was added to the system according to 1% of the wastewater treatment volume every day for a total of 15 days, and the treatment effect is shown in Table 2.

[0031] Table 2 Treatment effects of different salt-tolerant microbial agents

[0032]

[0033] It can be seen from Table 2 that after being treated with the combined microbial agent prepared by the present invention, the COD of the effluent is always lower than 60 mg / L. However, under the same conditions, ...

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Abstract

The invention relates to a microbial agent with tolerance to high-salt wastewater and capability of efficiently removing COD as well as a preparation method and application thereof. The microbial agent contains two or three of (Paracoccus sp.) FSTB-2, (Microbacterium kitamiense) FSTB-4 and (Pseudomonas stutzeri) FSTB-5. The three strains are preserved in China General Microbiological Center of Committee for Culture Collection of Microorganisms on June 1, 2015; the preservation numbers are CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940. The microbial agent can be directly used for treating COD in high-salt wastewater and can also be added into various biochemical reaction structures for improving microbiological compositions, optimizing salt tolerance of the microbial system in the wastewater treatment and increasing the overall COD removal rate of the process.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant microbial bacterial agent and its preparation method and application. The bacterial agent is especially suitable for efficient removal of COD in high-salt wastewater. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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