Power frequency electromagnetic field generating device having tumor inhibiting effect and loading method
A technology of tumor suppression and generation device, which is applied in the interdisciplinary field of physics, biology and medicine, to achieve the effect of uniform magnetic field strength, inhibition of growth and metastasis, and promotion of cell autophagy
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Embodiment 1
[0026] Embodiment 1, a kind of industrial frequency electromagnetic field generating device with tumor suppressing effect provided by the present invention, consists of an industrial frequency power supply 1, a first autotransformer 2 (alternating current AC), a second autotransformer 3 (direct current DC), The first diode bridge 4, the second diode bridge 5, the first group of coils 6, the second group of coils 7, the irradiation part 8, the voltage detection part 9, the magnetic field detection part 10, the temperature measurement part 11 and The terminal computer 12 is composed of a power frequency power supply 1 connected to one end of the first autotransformer 2 and the second autotransformer 3 respectively, and the other end of the first autotransformer 2 and the second autotransformer 3 are respectively connected to the first two poles The tube bridge 4 is connected with the second diode bridge 5, the other end of the first diode bridge 4 is connected with the first grou...
Embodiment 2
[0030] Example 2, the present invention acts on cultured Wilms tumor cell G401, using figure 1 and figure 2 device shown, according to the Figure 4 The program was used to treat the cells, each time the voltage was changed for 3.5 minutes, each round of action time was about 30 minutes in total, 4 rounds were treated every day, and the electromagnetic field irradiation time was 2 hours in total; the negative control group placed the cells between the coils that did not load the electromagnetic field The time is the same; the positive control is cisplatin (Jiangsu Hansoh Pharmaceutical Co., Ltd.), and the administration concentration is 500nM, once a day.
[0031] The specific implementation process is:
[0032] Cell line and culture method: Wilms tumor cell G401, which is an adherent cell, cultured in McCoy medium containing 10% fetal bovine serum in a 37°C incubator, containing 5% carbon dioxide and saturated water vapor;
[0033] Main equipment: power frequency electrom...
Embodiment 3
[0041] Example 3, the present invention acts on the cultured neuroblastoma cell CHLA255, using figure 1 and figure 2 device shown, according to the Figure 4 The program was used to treat the cells, each time the voltage was changed for 3.5 minutes, each round of action was about 30 minutes, 4 rounds were treated every day, and the electromagnetic field irradiation time was 2 hours in total; in the negative control group, the cells were placed between the coils without electromagnetic field loading. The same time was placed on the table; the positive control was cisplatin (Jiangsu Hansoh Pharmaceutical Co., Ltd.), the administration concentration was 500nM, and the administration was once a day.
[0042] The specific implementation process is:
[0043] Cell line and culture method: neuroblastoma cell CHLA255, which is an adherent cell, cultured in IMDM medium containing 10% fetal bovine serum in an incubator at 37°C, containing 5% carbon dioxide and saturated water vapor; ...
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