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PF-127-mirna-615 agomir complex and its preparation method and application

A pf-127-mirna-615agomir, 1. pf-127-mirna-615agomir technology, applied in the field of PF-127-miRNA-615agomir complex and its preparation, to achieve long effective action time, high miRNA activity, and reduced binding Effect

Active Publication Date: 2019-11-05
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the intracellular mechanism of its function is still unknown

Method used

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  • PF-127-mirna-615 agomir complex and its preparation method and application
  • PF-127-mirna-615 agomir complex and its preparation method and application
  • PF-127-mirna-615 agomir complex and its preparation method and application

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Effect test

Embodiment 1

[0034] Embodiment 1: Preparation of PF-127-miRNA-615 agomir complex

[0035] The PF-127-miRNA-615 agomir complex was prepared from Pluronic F-127 hydrogel, deionized water and miRNA-615 agomir by the following steps:

[0036] (1) Synthesis of miRNA-615 agomir:

[0037] In this example, miRNA-615 agomir is a miRNA-615 analog synthesized by special chemical modification, which was synthesized by Guangzhou Ruibo Biotechnology Co., Ltd.;

[0038] The basic information of miRNA-615 is shown in Table 1:

[0039] Table 1 Basic information of miRNA-615

[0040]

[0041]

[0042] The reaction mechanism between miRNA-615 and LINGO-1: Mature miRNA mainly affects the post-transcriptional translation efficiency and stability of genes by binding to the 3'-UTR of specific target genes. Mature miRNA-615 through its seed sequence (seedsequence)─ GGGGGUCCCC (Table 1 single underlined part) specifically binds to the partial sequence of the 3'-UTR of LINGO-1 mRNA (Table 1 double underli...

Embodiment 2

[0051] Example 2: Application of PF-127 encapsulating miRNA-615 agomir complex to repair brachial plexus root avulsion

[0052] 1. Preparation of rat model with brachial plexus root avulsion

[0053] Adult female SD rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (3.5ml / kg). Under a stereomicroscope, the right C4-T2 spinal cord segments were exposed, C4-C7 laminectomy was performed, and the right arm was separated. For the C5-C7 spinal nerve roots of the plexus, use a fine glass hook to gently tear off the C5-C7 nerve roots, and cut off a section of the spinal nerve connected to the C5 and C7 nerve roots, leaving a gap of about 5mm between the nerve and the spinal cord, and then remove the The anterior root of C6 was reset back to its original position, the bleeding was fully stopped, and the muscles and skin were sutured layer by layer intermittently. Keep warm after the operation, and send the animal to a clean animal breeding room for routine car...

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Abstract

The invention relates to the technical field of biomedicine, in particular to PF-127-miRNA-615 agomir complex and a preparation method and application thereof, wherein the PF-127-miRNA-615 agomir complex is made by covering miRNA-615 agomir with Pluronic F-127 hydrogel. A transporter capable of carrying miRNA-615 agomir and filling crack damage is provided, precise transport and local slow release of miRNA-615 agomir are achieved, and three-dimensional space and mechanical supporting capacity can be provided for the growth of neural axons. Therefore, the PF-127-miRNA-615 agomir complex can vigorously promote nerve regenerative repair for brachial plexus root avulsion, and new treatment routes are provided for central and peripheral nerve injury.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a PF-127-miRNA-615agomir complex and its preparation method and application. Background technique [0002] Brachial plexus avulsion (BPA) is a common disabling surgical trauma, especially total brachial plexus root avulsion, with high disability rate, poor curative effect and poor prognosis. With the continuous development of family motor vehicles and modern transportation and logistics, the incidence of brachial plexus injuries has increased year by year, and the most common causes are tractive injuries caused by traffic accidents and neonatal dystocia. The nerve root is composed of a central part and a peripheral part, and the junction part of the two is called the "transitional zone" (transitional zone, TZ). Brachial plexus avulsion is the physical separation of the nerve root and the spinal cord in the TZ region, which not only causes peripheral nerve rupture, but also l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7105A61K9/06A61K47/10A61P25/02A61L27/54A61L27/18A61L27/52
CPCA61K9/06A61K31/7105A61K47/34A61L27/18A61L27/52A61L27/54A61L2300/258A61L2300/60A61L2430/32C08L71/08
Inventor 吴洪福丁璐周光纪曾丽妮朱哲刘晓倩罗传铭邹堂斌孙雪荣邱文锋张文辉崔新月钟家贵
Owner GUANGDONG MEDICAL UNIV
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