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Model for inducing drug resistance in PA (pseudomonas aeruginosa) animal as well as construction method and application of model

A construction method and drug resistance technology, applied in the biological field, can solve the problems of inability to simulate the development law of Pseudomonas aeruginosa drug resistance, lack of Pseudomonas aeruginosa models, and inaccurate model evaluation standards.

Active Publication Date: 2017-05-10
BEIJING CHINESE MEDICINE HOSPITAL AFFILIATED CAPITAL MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention lies in the lack of Pseudomonas aeruginosa respiratory tract infection animal models in the prior art, which cannot simulate the development of Pseudomonas aeruginosa drug resistance in the airway of the body, and the model evaluation criteria are not accurate defects, thereby providing a PA animal in vivo drug resistance induction model and its construction method and application

Method used

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  • Model for inducing drug resistance in PA (pseudomonas aeruginosa) animal as well as construction method and application of model
  • Model for inducing drug resistance in PA (pseudomonas aeruginosa) animal as well as construction method and application of model
  • Model for inducing drug resistance in PA (pseudomonas aeruginosa) animal as well as construction method and application of model

Examples

Experimental program
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Effect test

Embodiment 1

[0072] This embodiment provides a method for constructing a drug resistance induction model in PA animals, including the following steps:

[0073] (1) Take 200 healthy ICR mice, half male and half female, weighing 14±1g, and randomly group them. The induction modeling period is set to 3 days, 5 days, 7 days, and 9 days, and each induction modeling period is blank. Control group, non-induced drug resistance model group A1, non-induced drug resistance model administration group A2, induced drug resistance model group B1 and induced drug resistance model administration group B2, a total of 20 groups (see Table 2 below), each group 10 for use; the non-induced drug resistance model group A1 and the non-induced drug resistance model administration group A2 are collectively called group A, and the induced drug resistance model group B1 and the induced drug resistance model drug administration group B2 are collectively called Group B;

[0074] (2) The mice in the A and B groups were anest...

Embodiment 2

[0101] In this example, on the basis of Example 1, the expression of the efflux pump gene mexC in mouse lung tissue is used to verify whether the drug resistance induction model in the PA animal is successfully constructed. Real-time fluorescent quantitative RT-PCR is used to detect The method for the expression of mexC gene includes the following steps:

[0102] 1) Collect the lung tissues of the mice in each group in Example 1 as samples, place them in a mortar, add liquid nitrogen, grind them, and collect them in a 1.5ml centrifuge tube. Add 1ml Trizol to each sample to extract RNA; Flick the bottom of the EP tube containing 1ml TrizolReagent to resuspend the mixed sample. Place it at room temperature for 20min, 4℃, 12000rpm, and centrifuge for 10min; transfer the clear supernatant into a new EP tube, add 0.2ml chloroform, and cover tightly. Cap the tube, shake vigorously for 15s, incubate at room temperature for 2-3min, 4℃, 12000rpm, centrifuge for 15min; transfer the superna...

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Abstract

The invention discloses a model for inducing drug resistance in a pseudomonas aeruginosa animal as well as a construction method and an application of the model. With the application of the model constructed by the method, better simulation of a phenomenon of PA infection in respiratory tract of a patient who accepts wide-spectrum antibiotics clinically for a long time can be achieved, and development and change rule of the drug resistance of pseudomonas aeruginosa in a mouse body can be observed, so as to simulate the change of the bacterium (the pseudomonas aeruginosa) in the body and to facilitate study on antibacterial drug-resistance medicine treatment; and meanwhile, the animal model, as a lung index evaluating animal model, is more intuitive, accurate in evaluating result, short in construction cycle and high in efficiency.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a Pseudomonas aeruginosa (PA) animal body drug resistance induction model and its construction method and application. Background technique [0002] Pseudomonas aeruginosa (PA), formerly known as Pseudomonas aeruginosa, is a non-fermenting gram-negative bacillus, and it is widespread in the skin, respiratory tract and intestine of normal people, and in the hospital environment. PA infection can cause a variety of diseases, such as burns, sepsis, intestinal infections, purulent infections of postoperative wounds, and respiratory tract infections. For patients with underlying diseases and long-term use of antibiotics and anti-tumor drugs, they are more likely to cause PA infectious pneumonia because of their weak or impaired immune function. [0003] The infection of PA is usually treated with antibiotics, but with the large-scale use of antibiotics, PA is very easy to develop res...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K31/5383A61K49/00A01K67/02
CPCA01K67/02A61K31/5383A61K35/74A61K49/0008
Inventor 刘清泉崔晓兰崔煦然郭玉红赵京霞丁军颖
Owner BEIJING CHINESE MEDICINE HOSPITAL AFFILIATED CAPITAL MEDICAL UNIV
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