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Production method, product, detection method and application of electrochemiluminescence immunosensor

A technique of luminescence immunity and electrochemistry, applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by chemical reaction of materials, etc. The effect of good performance, good stability and easy operation

Inactive Publication Date: 2017-04-26
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the quantum dot film prepared by embedding method will be affected by the swelling and steric hindrance effect of the film-forming material, and it is difficult to obtain a stable and strong electrochemiluminescent signal.

Method used

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  • Production method, product, detection method and application of electrochemiluminescence immunosensor
  • Production method, product, detection method and application of electrochemiluminescence immunosensor

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Embodiment 1

[0039] A preparation method of an electrochemiluminescence immunosensor based on CdTe@MOFs / CdTeQDs complex is as follows: comprising the following steps:

[0040] 1) Polish the glassy carbon electrode with a diameter of 4 mm with 0.3 and 0.05 μm alumina powder in turn, clean it with ultrapure water first, then ultrasonically wash it in ultrapure water, ethanol, and ultrapure water respectively, and dry it for later use ;

[0041] 2) Drop 5 μL of Au@TiO with a mass fraction of 24% on the surface of the electrode 2 The nanocomposite is dried at room temperature to obtain a modified electrode;

[0042] 3) Drop 15 μL of antibody with a mass fraction of 4% on the surface of the modified electrode, incubate at 4°C for 10-12 hours, and rinse with ultrapure water to obtain an immune electrode;

[0043] 4) Dropping 0.25% bovine serum albumin on the surface of the immune electrode to block the non-specific active sites, incubating at 37°C for 30 minutes, taking it out, washing with wa...

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Abstract

The invention discloses a production method, product, detection method and application of an electrochemiluminescence immunosensor based on CdTe@MOFs / CdTeQDs compound, and belongs to the field of electrochemiluminescence sensors. A metal-organic framework material is adopted to immobilize quantum dots, the special structure of the metal-organic framework material is used to immobilize the quantum dots in the metal-organic framework material and on the surface of the metal-organic framework material, the obtained composite material is used to immobilize a second antibody, a second antibody is immobilized under the assistance of a nano-gold functionalized titanium dioxide compound, and the obtained metal-organic framework material / quantum dot / antibody coupled compound and an antigen form a double-antibody sandwich immune reaction mode, so the identification and detection process of a target substance is realized, and the electrochemiluminescence signal is greatly enhanced. Effective detection of a biochemical marker is realized according to the intensity difference of the electrochemiluminescence signal of a substance-to-be detected with different concentrations.

Description

technical field [0001] The invention belongs to the field of electrochemiluminescence analysis and immunodetection, and in particular relates to a preparation method, product, detection method and application of an electrochemiluminescence immunosensor based on a CdTe@MOFs / CdTeQDs complex. Background technique [0002] Acute myocardial infarction is myocardial necrosis caused by acute and persistent coronary ischemia and hypoxia. Patients with this disease may have clinical symptoms such as heartache, arrhythmia, and heart failure. In severe cases, coma or shock may occur, and even life-threatening. Relevant studies have shown that effective treatment of patients with acute myocardial infarction within 6 hours of onset can achieve good therapeutic effects. Therefore, early diagnosis and treatment of patients with this disease are of great significance to save their lives. [0003] Cardiac troponin I (cTnI) is a subtype of cardiac troponin, and its content in the blood of n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N27/327G01N27/38G01N21/76
CPCG01N21/76G01N27/3275G01N27/38G01N33/6887
Inventor 卓颖梁文斌余燕清柴雅琴袁若
Owner SOUTHWEST UNIVERSITY
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