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Supermolecular sensor array and method for synchronously detecting paraquat and diquat

A sensor array and synchronous detection technology, applied in the field of pesticide detection, can solve the problems of high time and cost, inability to distinguish two analytes, etc., and achieve the effect of simple method and accurate results

Inactive Publication Date: 2017-04-26
CHINA AGRI UNIV
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Problems solved by technology

However, the instrumental analysis method requires the use of expensive instruments for identification and analysis after the samples are collected back to the laboratory, which is time-consuming and expensive, especially for ordinary people, who do not have the conditions to use complex and sophisticated instruments; and for chemical Sensor method, most sensors can only respond to one of the analytes or both can not distinguish between the two analytes

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  • Supermolecular sensor array and method for synchronously detecting paraquat and diquat

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Embodiment Construction

[0027] The present invention provides a supramolecular fluorescent sensor array and its application method for identifying and detecting paraquat and diquat, and the present invention will be further described below in combination with specific embodiments.

[0028] 1. Instruments and reagents

[0029] Varian Cary Eclipse Fluorescence Spectrophotometer.

[0030] Saffron T (ST), methylene blue (MB) and hematoxylin (HT) were purchased from Aladdin; carboxymethyl-β-cyclodextrin was purchased from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd.; paraquat and diquat The fast standard samples were all purchased from the Environmental Protection Scientific Research and Monitoring Institute of the Ministry of Agriculture, and diluted with ultrapure water to obtain a working solution of the required concentration.

[0031] 2. Design and recognition mechanism of supramolecular fluorescent sensor array

[0032] Compared to a single chemical sensor, a sensor array is a way to combine d...

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Abstract

The invention discloses a supermolecular sensor array and a method for synchronously detecting paraquat and diquat. The sensor array comprises probes 1, 2 and 3, wherein the probe 1 is a solution prepared from safranine T and carboxymethyl-beta-cyclodextrin; the probe 2 is a solution prepared from methylene blue and carboxymethyl-beta-cyclodextrin; the probe 3 is a solution prepared from hematoxylin and carboxymethyl-beta-cyclodextrin. According to the supermolecular sensor array, multiple probes are combined into the sensor array; a fluorospectrophotometer is further combined to be capable of carrying out qualitative and quantitative analysis on two analytes at the same time; the method is simple and is accurate in result.

Description

technical field [0001] The invention generally relates to the field of pesticide detection, in particular to the preparation of a supramolecular fluorescence sensor array and its application to simultaneous identification and analysis of paraquat and diquat. Background technique [0002] According to the announcements of the Ministry of Agriculture, the Ministry of Industry and Information Technology, and the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, the registration and production license of paraquat aqueous agents will be revoked and production will be suspended from July 1, 2014; Domestic sales and use. Diquat is the third largest herbicide in the world after glyphosate and paraquat. However, compared with paraquat, diquat has higher cost and limited weed killing spectrum. Therefore, in order to pursue profits, some manufacturers will sell paraquat products as diquat or illegally add paraquat to diquat pr...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 周志强刘畅刘东晖毕嘉玮王鹏
Owner CHINA AGRI UNIV
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