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Antithrombotic biological agent prepared by adopting nereis active peptide

A biological preparation and antithrombotic technology, applied in the preparation method of peptides, organic active ingredients, medical preparations containing active ingredients, etc., to achieve good stability, good medical application value, and increase the effect of economic added value

Active Publication Date: 2017-04-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extracting anticoagulant components from clam worms by enzymatic hydrolysis to inhibit thrombus in vivo has not been reported

Method used

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  • Antithrombotic biological agent prepared by adopting nereis active peptide
  • Antithrombotic biological agent prepared by adopting nereis active peptide
  • Antithrombotic biological agent prepared by adopting nereis active peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] An antithrombotic biological agent prepared by using nereis active peptide is prepared by mixing the following components in parts by weight: 10 parts of nereis anticoagulant peptide, 5 parts of heparin sodium and 100 parts of carrier.

[0045] The carrier is prepared by the following preparation method:

[0046] (1) chitosan is dissolved in 1vol% acetic acid solution to form 0.1wt% chitosan solution; gelatin is dissolved in 1vol% acetic acid solution to form 0.3wt% gelatin solution;

[0047] (2) Dissolve tea polyphenols in 0.1wt% chitosan solution, slowly add dropwise to 0.3wt% gelatin solution at 40°C, stir while adding dropwise, stirring speed 100r / min, continue to Stir for 60min. The mass ratio of tea polyphenols: gelatin: chitosan = 1:3:0.8.

Embodiment 2

[0049] An antithrombotic biological agent prepared by using nereis active peptide is prepared by mixing the following components in parts by weight: 20 parts of nereis anticoagulant peptide, 1 part of heparin sodium and 100 parts of carrier.

[0050] The carrier is the same as in Example 1.

Embodiment 3

[0052] An antithrombotic biological agent prepared by using nereis active peptide is prepared by mixing the following components in parts by weight: 15 parts of nereis anticoagulant peptide, 2 parts of heparin sodium and 100 parts of carrier.

[0053] The carrier is the same as in Example 1.

[0054] Preparation of clam worm anticoagulant peptide:

[0055] 1 Materials and methods

[0056] 1.1 Animals, materials and reagents

[0057] Healthy male clean-grade ICR mice, body weight (20±2) g; healthy male clean-grade SD rats, body weight (140±10) g, purchased from Zhejiang Academy of Medical Sciences, animal production license number: SCXK (Zhejiang )2014-0001.

[0058] The clam worm was purchased from Zhujiajian, Zhoushan, Zhejiang, and was identified by Professor Zhao Shenglong from Zhejiang Ocean University.

[0059] Trypsin, alkaline protease, neutral protease, papain, pepsin China Asia-Pacific Hengxin Company; fibrinogen, thrombin, carrageenan, ADP American Sigma Company;...

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Abstract

The invention discloses an antithrombotic biological agent prepared by adopting nereis active peptide. The antithrombotic biological agent is prepared by mixing the following components in parts by weight: 10-20 parts of nereis anticoagulant peptide, 1-5 parts of heparin sodium, and 100 parts of a carrier. The antithrombotic biological agent is good in stability and anticoagulation activity, has the antithrombus function, and has the good medical application value, and the economic additional value of nereis is increased.

Description

technical field [0001] The invention relates to an antithrombotic preparation, in particular to an antithrombotic biological preparation prepared by using nereis active peptide. Background technique [0002] Thrombosis is the common pathological basis of ischemic heart disease, ischemic stroke and venous thrombosis, which seriously endangers human health and has been increasing in recent years. Inhibition of platelet aggregation and release, inhibition of thrombin system, activation of antithrombin and plasmin system can inhibit thrombus formation. The formation of thrombus is the main pathological process of ischemic cardiovascular and cerebrovascular diseases and thromboembolic diseases, and is closely related to the occurrence and development of diseases. Therefore, inhibiting platelet aggregation and thrombus formation is of great significance to the prevention and treatment of cardiovascular and cerebrovascular diseases and other thromboembolic diseases. [0003] Hepa...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61K47/42C12P21/06C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16A61P7/02A61K31/727A61K35/64
Inventor 丁国芳艾杨洋黄芳芳杨最素李小娟许丹
Owner ZHEJIANG OCEAN UNIV
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