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Method and system for Y-STR typing of individual man

A Y-STR, individual technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as unreadable typing and typing crosstalk

Active Publication Date: 2017-03-22
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a method for Y-STR typing of male individuals, by adopting specific Y-STR locus combinations and specific primers designed for these loci, so that the typing will not be unreadable during the typing process , genotyping crosstalk, and dislocation problems, and can accurately obtain the genotypes of a total of 25 loci including 24 Y-STR loci and the sex-determining locus Amelogenin, so as to realize the family pedigree of the male individual Provide data support for screening, paternity identification, inference of the source of male components in mixed samples of men and women, and / or inference of sources of male components in mixed samples of men and women

Method used

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  • Method and system for Y-STR typing of individual man
  • Method and system for Y-STR typing of individual man
  • Method and system for Y-STR typing of individual man

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1. Verification of the accuracy of the method and system for Y-STR typing of male individuals of the present invention

[0060] In this example, the male individual is 193 male human anticoagulant blood samples, whose individual source is known, but in the implementation of Example 1 of this application, its individual source is assumed to be unknown, and the method and system of this application are used to analyze It performs Y-STR typing, including:

[0061] 1) Using the DNA acquisition system in the system of the present invention to obtain the DNA of male individuals, 2) Using the composite detection system in the system of the present invention to obtain the DNA including 24 Y-STR loci and the sex-determining locus Amelogenin The genotype of a total of 25 loci, and according to the genotype to obtain the individual's Y-STR typing result, 3) using the inference system described in the system of the present invention, according to the individual's Y-STR t...

Embodiment 2

[0089] Embodiment 2 Comparison between the detection system of the present invention and the detection system in the patent CN103866019

[0090] 1. In the detection system of the present invention and the patent CN103866019 detection system, the primers designed for each locus amplify the size range of the amplification products of each allele as follows:

[0091] Patent CN103866019:

[0092]

[0093] This application (which figure 1 The amplified product layout diagram of each locus of the application is shown):

[0094]

[0095] It can be seen from the black line that the amplification products of DYS460 and DYS389II in the patent CN103866019 are arranged too closely, which will cause the following two problems:

[0096] Question 1), the allelic typing standard (ladder) is incorrectly typed.

[0097] image 3 It shows the typing diagram of the allelic ladder (ladder) obtained by using the patent CN103866019 system. It can be seen that the shown patent CN103866019,...

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Abstract

The invention provides method and system for Y-STR typing of an individual man. The method comprises the following steps: acquiring the DNAs of the individual man, wherein acquired DNAs comprise genotypes of 25 gene loci including 24 Y-STR gene loci and a gender determination locus Amelogenin, and the 24 Y-STR gene loci are DYS460, DYS389I / II, DYS390, DYS392, DYS458, DYS437, DYS385a / b, GATA_H4, DYS522, DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635, DYS439, DYS19, DYS444, DYS527a / b, DYS617; and acquiring the Y-STR typing results of the individual man according to the 25 gene loci of the individual man. The Y-STR typing results can be used for family checking, male paternity testing, detection of male components in a hybrid male and female sample, detection of male components in a mixed sample of a plurality of males and the like for the individual man.

Description

technical field [0001] The present invention relates to a typing method and system, in particular to a method and system for Y-STR typing of male individuals. Background technique [0002] Tandem repeat sequence (short tandem repeat, STR) is a kind of polymorphic genetic marker system that widely exists in human chromosomal DNA. -7bp) and the length of the amplified products is less than 500bp, the number of the allele site represents the number of sequence repetitions. The allelic fragment lengths of STR loci are concentrated, so multiple STR loci can be amplified multiple times. Multiple STR loci are compounded and amplified, and the cumulative identification ability can approach or reach the level of DNA fingerprinting. [0003] The Y chromosome is a male-specific chromosome, and all male individuals of the same paternal line have the same Y-STR haplotype. As a supplementary method of autosomal testing, Y-STR test has important application value for family investigatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2525/151
Inventor 莫晓婷张建孙敬孙启凡马温华赵兴春叶健
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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