Micro-nano material, product of covalent modification of hydrophilic substances for surface of micro-nano material, and preparation method

Abstract

The invention discloses a micro-nano material, a product of covalent modification of hydrophilic substances for the surface of the micro-nano material, and a preparation method. Carboxyl groups or potential carboxyl groups are contained on the surface of the micro-nano material and transformed into active esters; the product of covalent modification of the hydrophilic substances for the surface is obtained through taking a hydrophilic compound or / and a hydrophilic polymer containing aliphatic primary amines or / and aliphatic secondary amines as a modifier, forming amido bonds from the active esters for the surface and the hydrophilic compound or / and the hydrophilic polymer, and carrying out covalent modification; during preparation, enough carboxyl groups or potential carboxyl groups are generated on the surface of a polymeric material from monomers containing carboxyl groups or / and potential carboxyl groups and transformed into active esters, then amido bonds are formed from the active esters and a modifier with aliphatic primary amines and / or aliphatic secondary amines, potential facultative ionic groups and hydrophilic connection arms, and having a moderate volume, and covalent modification layers are obtained; and multilayer covalent modification is realized during a process of repeatedly generating the active esters on the surface of the product of covalent modification and then forming the amido bonds from the active esters and the hydrophilic modifier, and the surface of the micro-nano material is efficiently covered with the hydrophilic modifier having a medium volume, thus the non-specific adsorption of the modification product for biological molecules is remarkably reduced.

Description

technical field [0001] The invention relates to a micro-nano material, a product covalently modified with a hydrophilic substance on the surface and a preparation method; the essence belongs to biomedical micro-nano material and its surface modification technology. Background technique [0002] In the present invention, substances with a molecular weight of less than 500 Daltons are called compounds, and substances with a molecular weight of not less than 500 Daltons are called polymers. [0003] Modification of the surface of micro-nano materials with hydrophilic compounds / polymers can reduce their non-specific adsorption and enhance applications, such as polyethylene glycol (PEG), zwitterionic substances, hydrophilic natural biopolymers (heparin and albumin) etc.) to modify. Natural biopolymers are prone to non-specific adsorption on other biomolecules, and still have strong non-specific adsorption on hydrophobic small molecules. On the contrary, PEG and zwitterionic com...

AI Technical Summary

Problems solved by technology

[0009] In order to ensure that the re-modification step after each layer of modification reaction has a sufficiently high degree of modification, there must be enough carboxyl groups in the modifier used in each modification reaction, but the electrostatic repulsion between these carboxyl groups will reduce the interaction between the modifier itself and the surface of the micro-nano material. Reactivity and degree of modification of reactive esters

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