A composition containing maca and leek seeds, its preparation method and application
A technology of leek seeds and a composition is applied in the field of compositions containing maca and chive seeds and the preparation thereof, which can solve the problems of poor efficacy, pollute the environment and the like, and achieve the effects of low cost, no organic residues and good safety.
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Embodiment 1
[0019] After crushing maca and chive seeds respectively, weigh maca and chive seeds with a mass ratio of 0.5:1-3:1 and put them into supercritical CO 2 In the extractor, set extraction conditions, including: extraction pressure 30-40MPa, extraction temperature 40-55°C, analysis pressure 6-13MPa, analysis temperature 45-60°C, and collect the extract.
Embodiment 2
[0021] On the basis of Example 1, after maca and chive seeds were pulverized respectively, the maca and chive seeds were weighed with a mass ratio of 1:1 and charged with supercritical CO 2 In the extractor, set extraction conditions, wherein: extraction pressure 30-40MPa, extraction temperature 40-55°C, analysis pressure 6-13MPa, analysis temperature 45-60°C, collect the extract as the composition of the present invention.
Embodiment 3
[0023] According to the method of embodiment 2, the Maca after the pulverization and leek seed are respectively subjected to supercritical CO 2 Extraction, set the extraction condition identical with embodiment 2, collect the supercritical CO of Maca, leek seed respectively 2 extract, and then the two extracts were mixed according to 1:1, as a control.
[0024] Select SPF grade Kunming mice, male, aged 9 to 11 weeks, 30 mice, randomly divided into blank group, control group, composition group, 10 mice in each group, the test dose is 24mg / kg, intragastric administration every day 1 time. The blank group was replaced by distilled water, the control group was administered with the same amount of the control substance of this example, and the composition group was administered with the same amount of the composition in Example 2, for 14 days in a row, free to move. Another selection of SPF grade Kunming mice, female, aged 9 to 10 weeks, 60 mice, subcutaneously injected estradiol...
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