Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sperm electron microscopic sample slide preparation method

An electron microscope and specimen technology, which is applied in the field of sperm electron microscope specimen preparation, can solve the problems of sperm reagent dispersion and production failure, and achieve the effects of improving success rate, avoiding waste, and improving work efficiency

Inactive Publication Date: 2017-03-15
ZUNYI MEDICAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to provide a method for making slices of sperm electron microscope specimens, so as to solve the technical problem that in the process of preparing sperm specimens in the prior art, the exposed sperm are easily washed away by reagents, resulting in the failure of making slices

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for preparing a sperm electron microscope specimen, comprising the following steps:

[0014] a. Asphyxiate the experimental mice by means of experiments, soak the experimental mice in 75% alcohol for sterilization, and cut a V-shaped incision in the abdomen of the experimental mice to expose the epididymis; separate the epididymis, and remove the epididymis from the experimental mice Take out the abdomen and wash it with PBS until the liquid is clear and there is no blood residue. Cut the epididymis short, keep the tail of the epididymis, and soak it in 2.5% glutaraldehyde for 2 hours;

[0015] b. Place the cauda epididymis treated in step a under the environment of 5° C. for 26 hours, and trim it into a strip-shaped electron microscope specimen with a size of 1 mm×1 mm×2 mm for electron microscope observation.

Embodiment 2

[0017] A method for preparing a sperm electron microscope specimen, comprising the following steps:

[0018] a. Asphyxiate the experimental mice by means of experiments, soak the experimental mice in 75% alcohol for sterilization, and cut a V-shaped incision in the abdomen of the experimental mice to expose the epididymis; separate the epididymis, and remove the epididymis from the experimental mice Take out the abdomen and wash it with PBS until the liquid is clear and there is no blood residue, cut the epididymis short, keep the tail of the epididymis, and soak it in 2.5% glutaraldehyde for 1.5 hours;

[0019] b. Place the cauda epididymis treated in step a under the environment of 4° C. for 24 hours, and trim it into a strip-shaped electron microscope specimen with a size of 1 mm×1 mm×2 mm for electron microscope observation.

Embodiment 3

[0021] A method for preparing a sperm electron microscope specimen, comprising the following steps:

[0022] a. Asphyxiate the experimental mice by means of experiments, soak the experimental mice in 75% alcohol for sterilization, and cut a V-shaped incision in the abdomen of the experimental mice to expose the epididymis; separate the epididymis, and remove the epididymis from the experimental mice Take out the abdomen and wash it with PBS until the liquid is clear and there is no blood residue, cut the epididymis short, keep the tail of the epididymis, and soak it in 2.5% glutaraldehyde for 1 hour;

[0023] b. Place the cauda epididymis treated in step a under the environment of 3° C. for 20 hours, and trim it into a strip-shaped electron microscope specimen with a size of 1 mm×1 mm×2 mm for electron microscope observation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a sperm electron microscopic sample slide preparation method in the field of microscope slides preparation. The method comprises the following steps: a, killing a laboratory rat, sterilizing by immersing the laboratory rat in 75% alcohol, cutting the laboratory rat in the abdomen to expose epididymis; separating epididymis, taking the epididymis out of the laboratory rat's abdomen, washing with PBS liquor until the liquor is clear and there is no residual blood, cutting the epididymis short, retaining tail of epididymis, and immersing (fixing) the tail of epididymis in 2.5% glutaraldehyde; and b, placing the processed tail of epididymis in an environment of 3-5 DEG C for 20-26h, and making the electron microscopic sample. According to the scheme, the sperm is avoided from being directly contacted with any reagent used in the slide preparation process. Thus, the sperm is prevented from being dispersed by the reagent, success rate of the slide preparation is raised, wastes of various raw materials used in the slide preparation process are avoided, and work efficiency is also enhanced.

Description

technical field [0001] The invention relates to the field of preparation of microscope slides, in particular to a method for preparing sperm electron microscope specimens. Background technique [0002] Due to the weak penetrating ability of the electron beam generated by the electron microscope, it is necessary to cut the specimen into thin slices with a thickness of less than 0.1 μm. The ultrathin section technique is the most commonly used and basic preparation technique for preparing ultrathin sections. The production process basically needs to go through steps such as material collection, fixation, dehydration, soaking, embedding polymerization, sectioning and staining. [0003] In laboratory experiments, in order to reduce the interference of uncontrollable factors, the observation target is usually processed in a targeted manner to avoid inclusion of other interference factors. For example, in the experimental study of male reproductive ability, it is often necessary...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 孔德营张继东张仕斌刘福慧
Owner ZUNYI MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com