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Molecular marker combinations, methods and applications for rapid identification of different albino tea tree varieties

A technology of albino tea trees and molecular markers, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of unsuitable seedlings, buying false seedlings, uncertain which species the tea seedlings are, etc. problems, to achieve the effect of convenient product information and simple identification methods

Active Publication Date: 2019-09-24
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase in the number of newly bred albino tea tree varieties, and the higher prices of seedlings and finished tea for new varieties, many unscrupulous traders have begun to take advantage of the characteristics of difficult identification of tea and tea seedling varieties, and replace old ones with new ones and inferior ones as good ones.
After investigation, it was found that many tea farmers are often not sure which type of tea seedlings they buy, and they buy fake seedlings or inappropriate seedlings; there are also many tea processing companies that are not sure which type of finished tea they process comes from. , thus unable to provide consumers with more accurate product information
At present, there is no effective means to identify and distinguish the specific varieties of different albino tea seedlings or finished teas.

Method used

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  • Molecular marker combinations, methods and applications for rapid identification of different albino tea tree varieties
  • Molecular marker combinations, methods and applications for rapid identification of different albino tea tree varieties
  • Molecular marker combinations, methods and applications for rapid identification of different albino tea tree varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening experiment of molecular marker combination

[0027] 1 Materials and methods

[0028] 1.1 Materials

[0029] As shown in 16 kinds of albino tea tree samples (see Table 1) for testing, the samples are all collected from the National Tea Tree Germplasm Resource Garden Hangzhou Branch. The new shoots with one bud and two leaves of each test sample without infection by diseases and insect pests were picked, and after being quickly frozen in liquid nitrogen, they were stored in an environment of -80°C for later use.

[0030] Table 1 The number and name of the test samples

[0031]

[0032]

[0033] 1.2 Method

[0034] 1.2.1 Genomic DNA extraction method

[0035] All samples were ground into powder in liquid nitrogen, genomic DNA was extracted by CTAB method, the quality of DNA was detected by 1% agarose gel electrophoresis, and the DNA concentration was measured by ND-1000 micro-volume ultraviolet spectrophotometer, and then the DNA concentration...

Embodiment 2

[0061] Example 2 A method for rapidly identifying different albino tea tree varieties using molecular marker combinations

[0062] The three pairs of primers screened in Example 1 can be used to identify the main albino tea tree varieties currently on the market, thereby solving the problem of mixed names of tea tree albino seedlings on the market.

[0063] For example, when tea farmers or tea seedling breeding enterprises do not know the background information of a certain albino tea tree variety at all, they can use the three pairs of primers CSR238, CSR1297, and LG04-06 screened out in this study to identify the variety. The specific identification method is as follows:

[0064] (1) DNA is extracted from the fresh young leaves of the tea tree species to be tested or the finished tea;

[0065] (2) Using the DNA extracted in step (1) as a template and using CSR238, CSR1297, and LG04-06 as primers to carry out PCR amplification respectively;

[0066] (3) Carry out electrophor...

Embodiment 3

[0070] Embodiment 3 Application of Fingerprint Two-Dimensional Code

[0071] The tea sales enterprise can use the two-dimensional code generated in Embodiment 1 of the present invention to mark the products processed by the company's different albino tea tree varieties, so that consumers can understand product information more conveniently.

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Abstract

The invention discloses a molecular marker combination for rapidly identifying different albino tea tree varieties. The molecular marker combination adopts CSR238, CSR 1297 and LG04-06 primer combinations; the invention further discloses a method for rapidly identifying different albino tea tree varieties by using the molecular marker combination and a kit containing the molecular marker. The molecular marker combination can distinguish and identify the albino tea tree varieties on the market, and the identifying method is simple and fast, and can solve the problem of confused names of albino tea tree varieties on the market for a long time; the invention further discloses a finger-print of the albino tea tree varieties; tea selling enterprises can apply the two-dimensional code albino tea tree varieties generated by the invention to mark the product formed by processing different albino tea tree varieties, thus consumers can more conveniently learn about the product information.

Description

technical field [0001] The invention relates to the field of tea tree biotechnology, in particular to a molecular marker combination, method and application for rapidly identifying different albino tea tree varieties. Background technique [0002] White tea varieties are a kind of leaf color mutants with different degrees of chlorophyll deficiency produced under specific conditions. They are rare resources in tea trees and are currently found all over the country, such as Anji white tea, Jingning white tea, and golden buds in Zhejiang. ; Jiangxi golden chrysanthemum and so on. Existing studies have shown that due to the specificity of the metabolic mechanism of this kind of tea tree resources, the chlorophyll synthesis of the new buds and leaves is reduced, and the carbon metabolism is inhibited, so that the buds and leaves show different degrees of albinism. The production of free amino acids is improved, so that the content of free amino acids in some varieties of resourc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 马春雷陈亮黄丹娟姚明哲王松琳马建强金基强
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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