Method and system for realizing multi-point light focusing and light spot optimization at any position

Abstract

The invention discloses a method and a system for realizing multi-point light focusing and light spot optimization at any position. The method for realizing multi-point light focusing and light spot optimization at any position comprises the following steps: under the condition that no samples are loaded, carrying out graded distribution phase modulation focusing on light beams, thus obtaining ideal focusing light spots; after the samples are loaded, enabling the light beams to be focused to a scattering medium, thus obtaining distortion scattering light spots; partitioning an image of a spatial light modulator, loading phase light focusing to each subarea, thus obtaining corrected scattering light spots, and carrying out once correcting cycle, thus obtaining new modulation phase distribution; loading the new modulation phase distribution to a spatial light modulator, carrying out modulation, carrying out light focusing again, thus obtaining the corrected light spots after the correcting cycle, and carrying out cross correlation calculation with the ideal focusing light spots, thus obtaining a cross correlation coefficient; and carrying out a plurality of times of circular correcting through iteration till the cross correlation coefficient after correcting cycle is greater than a preset coefficient threshold value. With the method and the system, the distribution of the scattering light spots is optimized, the limitation that the existing optogenetics photostimulation and microimaging scheme only can realize the stimulation at the single area is broken through, and the multi-area photostimulation and microimaging can be simultaneously realized under the great depth inside the brain.

Description

technical field [0001] The invention belongs to the field of light stimulation and microscopic imaging of optogenetic technology, in particular to a method and system for multi-point light focusing at any position and spot optimization, and is applied to microscopic imaging. Background technique [0002] Optogenetics technology—genetically encoding light-sensitive proteins that activate signaling pathways and replacing endogenous electrical activity with custom action potential excitation patterns—is a combination of optical and genetic methods to precisely control method of cellular activity. The revolution in neurology brought about by optogenetics technology has transformed the way we study neural circuits. Neuroscience researchers can intuitively study the relationship between individual neurons and multiple nuclei by directly manipulating neurons. Intrinsic interaction to further intuitively elucidate the structure, function and behavior of brain neural circuits. Usin...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to use the limitation that the existing optogenetic stimulation scheme can only stimulate a single area, and provide a multi-point light focusing and light spot optimization at any position by combining the spatial light modulator and adaptive optics technology. method and system

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