Luminous bacterium flow injection method for quickly detecting and warning food-borne toxin pollution and application of luminous bacterium flow injection method

Abstract

The invention belongs to the field of food safety, environment monitoring and public safety, and particularly discloses a luminous bacterium flow injection method for quickly detecting and warning food-borne toxin pollution and application of the luminous bacterium flow injection method. The luminous bacterium flow injection method includes steps of resuscitating, inoculating and continuously cultivating vibrio qinghaiensis Q67 microbial agents; designing flow injection system programs; detecting sample solution by the aid of luminous bacterium flow injection processes; integrating and processing data and designing warning systems. The luminous bacterium flow injection method and the application have the advantages that the food-borne toxin pollution can be detected and warned by the aid of the created luminous bacterium flow injection method, the luminous bacterium flow injection method is high in analysis speed and automation degree, easy and convenient to implement and good in repeatability, and errors due to manual operation can be prevented; flow injection analysis processes are combined with luminous bacterium continuous cultivation, and accordingly the possibility of cross pollution is extremely low; vibrio qinghaiensis is used as an indicator for measuring pollution toxin and is wide in detection range and sensitive in reaction.

Description

technical field [0001] The invention belongs to the fields of food safety, environmental monitoring and public safety, and in particular relates to a flow injection luminescent bacteria method for rapid detection and early warning of food-borne toxin pollution and its application. Background technique [0002] In the sudden drinking water pollution incidents that have occurred in recent years, it is difficult to detect the poisoning of drinking water in time. Some common food-borne toxins are highly toxic and easy to obtain, and are easy to be used by criminals. The rapid detection and early warning system of foodborne toxins is of great significance. [0003] Chemical analysis can be used for qualitative and quantitative analysis of pollutants in the water environment, but it cannot directly reflect the impact of pollutants on the environment and organisms. The toxicity test method using luminescent bacteria as the test organism has attracted attention because of its rapid...

AI Technical Summary

Problems solved by technology

In recent years, Vibrio qinghai has received widespread attention, but the matching methods are still not uniform, and most of the bacterial freeze-dried powder is resuscitated and luminescent to directly detect water quality toxicity

However, there are the following problems in the recovery method of bacterial freeze-dried powder: first, the production cost of freeze-dried powder is high; second, the difference in recovery rate will affect the test results; third, it cannot be carried at room temperature

The above characteristics limit its popularization and application

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