ITS (internal transcribed spacer) sequence of dalbergia odorifera and method for identifying dalbergia odorifera by ITS sequence

Abstract

The invention discloses an ITS (internal transcribed spacer) sequence of dalbergia odorifera and a method for identifying the dalbergia odorifera by the ITS sequence. The ITS sequence of the dalbergia odorifera is characterized in that a nucleotide sequence is shown in SEQ ID NO:1. The method comprises the following steps of using primers ITS4 and ITS5 to perform PCR (polymerase chain reaction) proliferation on DNA (deoxyribonucleic acid) of a to-be-tested wood sample, so as to obtain ITS sequences; matching and comparing the two ITS sequences, and checking basic group information of ten sites, so as to judge whether the to-be-tested wood sample is the dalbergia odorifera or not. The method has the advantages that the dalbergia odorifera can be simply, quickly and efficiently subjected to molecular identification at the molecular biological level; the cost is low, the operation is simple, and the identification accuracy is high.

Description

technical field [0001] The invention relates to plant species identification technology, in particular to the identification technology of Dalbergia rosewood, in particular to an ITS region sequence of Dalbergia japonica and a method for identifying Dalbergia japonica using the ITS region sequence. Background technique [0002] Mahogany is a general term for a class of wood used to make mahogany furniture and mahogany crafts. According to the provisions of the national standard GB / T 18107-2000 "Redwood" of the People's Republic of China, there are 5 genera (Red Sandalwood, Dalbergia, Jasmine, Iron Sword Wood, Persimmon) and 8 categories (Red Sandalwood, Rosewood, etc.) 33 types of wood, fragrant wood, black rosewood, red rosewood, chicken wing wood, ebony, striped ebony). Among them, Dalbergia Dalbergia ( Dalbergia odorifera T. Chen) is an endangered species in Hainan and is a national second-class protected plant. It is commonly known as Hainan Xiangzhimu or Hainan Huang...

AI Technical Summary

Problems solved by technology

[0003] Traditional mahogany identification and distinction are mainly based on macroscopic characteristics (such as density, structure, material color and texture, etc.), supplemented by necessary wood microanatomical methods to determine its genus and species. Wood morphology and anatomy knowledge and identification experience, and because of the similar morphological characteristics between closely related species, traditional morphological and anatomical identification methods are usually difficult to accurately identify wood to the level of species, which brings great difficulties to the identification of mahogany , unable to meet market demand

Shen Mingyue et al. (2012) used high-performance liquid chromatography (HPLC), Zhu Tao et al. (2013) used gas chromatography-mass spectrometry (GC-MS) to successfully identify species of redwood, but these methods are not Equipment and other hardware requirements are high and limitations are high