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Preparation method and application of microbial flocculant

A technology of microbial flocculant and inoculation amount, which is applied in chemical instruments and methods, biological water/sewage treatment, water pollutants, etc., can solve the problems of harsh culture conditions, difficult to settle, difficult to cultivate, etc., to achieve good flocculation effect and not easy to Contaminated, short processing time effect

Inactive Publication Date: 2017-02-22
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is: to solve the technical problems of microbial flocculants in the prior art, or the cultivation conditions are harsh, difficult to cultivate, or the flocs produced are small and difficult to settle, and provide A preparation method of a microbial flocculant that has good flocculation effect, can produce larger flocs during the flocculation process, and can settle rapidly by itself. The method is simple to operate and low in cost

Method used

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  • Preparation method and application of microbial flocculant
  • Preparation method and application of microbial flocculant
  • Preparation method and application of microbial flocculant

Examples

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Embodiment 1

[0029] The determination of embodiment 1 target bacterial strain

[0030]In this example, 9 kinds of microbial flocculant producing bacteria were screened and isolated from the sludge of the secondary sedimentation tank of a printing and dyeing company in Chongqing, numbered C-1~C-9, and the strains of each number were fermented separately, and the results showed the number The flocculation rate of the fermentation supernatant of the C-6 strain to the kaolin suspension is 95.46%, and the flocculation activity is very stable, which is suitable for use as a microbial flocculant. The morphological identification and ITS rDNA sequence determination of the C-6 strain were carried out, and the BLAST sequence comparison of the NCBI database (see Appendix 1 for the sequence) identified the C-6 strain as Aspergillus niger. The results are shown in Figure 1~4 ( Figure 4 Middle M lane is Marker 2000, NC lane is negative control, 1 lane is C-6 strain), it can be seen that the bacterial...

Embodiment 2

[0031] The fermentation of embodiment 2 Aspergillus niger enzyme

[0032] The fermentation conditions of Aspergillus niger were optimized to make the flocculation effect of the fermented product better. The results showed that the medium solvent for microbial flocculants produced by Aspergillus niger was water, and the solutes included the following components: 15-50 g·L -1 Glucose, 0.1~0.5g·L -1 NaCl, 0.2~1.0 g·L -1 (NH 4 ) 2 SO 4 , 0.5~1.0 g·L -1 Urea, 0.5~1.0 g·L -1 Yeast extract, 0.4~0.6 g·L -1 MgSO 4 ·7H 2 O, 5.0 g L -1 K 2 HPO 4 and 2.0 g L -1 K H 2 PO 4 . The fermentation culture conditions are as follows: inoculate Aspergillus niger with an inoculation amount of 2.0~4.0% (V / V) on the medium with an initial pH of 6.0~9.0, at 30~35°C, 150~200r·min -1 Shaking culture under the conditions of 36 ~ 72h.

[0033] Further research shows that the optimal medium solvent for microbial flocculants produced by Aspergillus niger is water, and the solute includes ...

Embodiment 3

[0035] Example 3 Purification of Fermentation Products

[0036] The fermentation broth was subjected to the following steps (see Figure 5 ) is extracted and purified into a powder product (named MBFC-6). The specific method is to collect the fermentation broth fermented and cultivated for 52 hours under the best fermentation conditions in Example 2, and through 3000r min -1 Centrifuge for 30 minutes to remove bacteria (cells) to obtain the fermentation supernatant, use a rotary evaporator to concentrate the supernatant to 0.5 times the original volume at a temperature of about 45°C, take out the concentrated fermentation supernatant and add 2 times volume of pre-cooled absolute ethanol, and put it in a refrigerator at 4 ℃ for 24 hours, and the solid precipitate was precipitated by 5000 r min -1 Collect by centrifugation for 30 minutes, and dry in vacuum to obtain the crude product of microbial flocculant MBFC-6. Then dissolve the crude microbial flocculant MBFC-6 in distill...

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Abstract

The invention provides a preparation method and an application of a microbial flocculant. The method comprises steps as follows: a culture medium with initial pH being 6.0-9.0 is inoculated with aspergillus niger with inoculum concentration of 2.0%-4.0% (V / V), the aspergillus niger is subjected to shaking culture for 36-72 h under the condition of 30-35 DEG C and 150-200 r.min<-1>, fermentation broth is collected, and the microbial flocculant is obtained. The microbial flocculant can be used for performing flocculent precipitation on suspended solids in wastewater and can be combined with a solid adsorbent to decolorize dye wastewater. The microbial flocculant is non-toxic, biodegradable and free of secondary pollution, and the flocculation rate of the suspended solids in the wastewater is up to 90% or higher; through combination with modified montmorillonite, the decolorization ratios of methylene blue wastewater and indigo blue wastewater are up to 99.09% and 91.00% respectively, and unexpected flocculent precipitation and decolorization effects are realized.

Description

technical field [0001] The invention belongs to the field of flocculant technology, and in particular relates to a preparation method and application of a microbial flocculant. Background technique [0002] Among the water treatment methods, the flocculation method is the most widely used method in water treatment at home and abroad because of its advantages of good treatment effect, low cost and easy operation. The flocculation method is to add a certain amount of flocculant to the wastewater system to be treated, so that the inorganic and organic pollutants in the form of colloids and suspended solids in the water contact, collide, destabilize, Condensed into aggregates of a certain particle size, and the destabilized aggregates form large flocs (alum flowers) visible to the naked eye through further collision, chemical bonding, net sweeping, co-precipitation, etc., and finally rely on gravity Separation of solid and liquid to complete the flocculation process, thereby re...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F1/28
CPCC02F3/34C02F1/281C02F2101/308
Inventor 罗平杨林玉贾晓燕陆小琴卫宏毅
Owner CHONGQING UNIV
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