Traditional Chinese medicine compound prescription for simultaneously resisting swine infuenza and porcine reproductive and respiratory syndrome and extraction method thereof
A technology for blue ear disease and swine flu, applied in antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as the lack of research on compound prescriptions and the inability to comprehensively evaluate the effects of porcine reproductive and respiratory syndrome virus , to achieve the effects of not being resistant to drugs, regulating the immune function of the body, and improving clinical symptoms
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Embodiment 1
[0047] The in vitro anti-porcine reproductive and respiratory syndrome virus (PRRSV) effect of embodiment 1 Chinese medicine compound preparation
[0048] 1. Determination of the median lethal dose (TCID50) of PRRSV on MARC‐145 cells
[0049] The virus to be tested was serially diluted 10 times in 10 concentration gradients, and the virus solution was added to a 96-well plate full of Marc-145 cells at a dose of 100 μl / well. Each gradient was repeated 8 times, and the last 2 columns were cells. For the control group, the virus liquid was discarded after adsorption at 37°C for 1.5 h, and 100 μl of maintenance liquid was added, and cultured in a CO2 cell incubator.
[0050] The cell lesion was observed every day, and the OD value of each well was measured by the MTT method after the lesion reached 75%. Growth inhibition rate of virus on cells=(OD value of cell control group-OD value of virus control group) / OD value of cell control group. The median toxic concentration (TCID50) ...
Embodiment 2
[0070] Example 2 Chinese medicine compound prevents and treats the mice infected with H1N1 and H3N2 subtype swine influenza virus
[0071] 1. H1N1 and H3N2 subtype swine influenza virus to chicken embryo half infectious dose (EID 50 ) determination
[0072] The virus liquid was sequentially diluted 10 times, and inoculated in the allantoic cavity of 10-day-old chicken embryos, each dilution was inoculated with 0.2ml / piece, and 4 replicates were made, and normal chicken embryos were set as a control at the same time. Place them in an incubator at 37°C for 72 hours, discard the dead embryos within 24 hours, harvest the allantoic fluid of the dead embryos after 24 hours and live embryos after 72 hours, and measure the titer of HA. Calculate EID according to the Reed-Muench method 50 .
[0073] 2. Effect of traditional Chinese medicine compound on pneumonia in mice infected with H1N1 and H3N2 subtype swine influenza virus
[0074] (1) Grouping of experimental animals
[0075]...
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